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Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
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OverviewOxygenic photosynthesis plays a central role in the global carbon and oxygen cycles. The carbohydrates produced support nearly all food webs, while the oxygen by‑product enables aerobic life.Light‑dependent and light‑independent reactionsPhotosynthesis occurs in two main stages, each in a different part of the chloroplast: light‑dependent reactions and light‑independent reactions, also called the Calvin‑Benson cycle or simply the Calvin...
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Cellular respiration is a fundamental metabolic process that enables organisms to generate energy from organic molecules. One of its central pathways is the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle, which plays a crucial role in energy production and biosynthetic processes.Conversion of Pyruvate to Acetyl-CoAThe pyruvate generated from glycolysis undergoes oxidative decarboxylation by the pyruvate dehydrogenase complex, producing acetyl-CoA, one molecule of NADH, and one...
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Related Experiment Video

Updated: Nov 3, 2025

Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis
10:46

Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis

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Photorespiration: The Futile Cycle?

Xiaoxiao Shi1, Arnold Bloom1

  • 1Department of Plant Sciences, University of California Davis, Davis, CA 95616, USA.

Plants (Basel, Switzerland)
|June 2, 2021
PubMed
Summary

Photorespiration (C2 photosynthesis) is vital for plant processes, not just wasteful. Manganese cofactor can improve energy efficiency in these reactions.

Keywords:
atmospheric CO2climate changecrop yieldkineticsmetabolic interactionsmetal cofactoroxygenationphotorespirationphotosynthesis

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Area of Science:

  • Plant Physiology
  • Biochemistry
  • Photosynthesis Research

Background:

  • Photorespiration (C2 photosynthesis) is often viewed as a detrimental process reducing carbon fixation by over 25%.
  • Despite this perception, photorespiration is crucial for essential metabolic pathways including nitrogen and sulfur assimilation.
  • Current research predominantly uses magnesium as the primary metal cofactor, overlooking manganese's role.

Purpose of the Study:

  • To review methods for quantifying photorespiration.
  • To examine the impact of metal cofactors, particularly manganese, on photorespiratory enzymes.
  • To re-evaluate the perceived inefficiency of photorespiration.

Main Methods:

  • Literature review of photorespiration quantification techniques.
  • Analysis of existing research on metal cofactor influence on photorespiratory enzymes.
  • Synthesis of data to discuss the functional significance of photorespiration.

Main Results:

  • Manganese, often overlooked, is readily associated with key photorespiratory enzymes.
  • The presence of manganese can enhance the energy efficiency of these enzymatic reactions.
  • Established methods for photorespiration measurement are summarized.

Conclusions:

  • Photorespiration plays indispensable roles in plant metabolism beyond carbon fixation.
  • Manganese cofactor significantly influences the efficiency of photorespiratory enzymes.
  • The traditional view of photorespiration as a wasteful process requires re-evaluation.