Olive Oil with Ozone-Modified Properties and Its Application

Area of Science:

• Cosmetic Science
• Organic Chemistry
• Microbiology

Background:

• Olive oil is a common cosmetic ingredient, but its stability can be a limitation.
• Ozonation is a process that can modify oil properties.
• Investigating ozonated olive oil for cosmetic applications could lead to improved formulations.

Purpose of the Study:

• To evaluate the chemical and biological properties of ozonated olive oil.
• To assess the performance of cosmetic emulsions formulated with ozonated olive oil.
• To determine the potential of ozonated olive oil as a raw material in cosmetics and pharmaceuticals.

Main Methods:

• Olive oil was ozonated at two different concentrations (0.04 and 0.10 mole O3/100 g oil).
• Chemical composition was analyzed using gas chromatography-mass spectrometry (GC-MS) and headspace solid-phase microextraction (HS-SPME) GC-MS.
• Antimicrobial activity and cytotoxicity were assessed using agar diffusion and MTT assays, respectively.
• Cosmetic emulsions were prepared and evaluated for organoleptic properties, preservative efficacy, and stability over six months.

Main Results:

• Ozonation decreased unsaturated fatty acids but introduced new compounds enhancing physicochemical and sensory properties.
• Emulsions based on ozonated olive oil showed improved stability and longer shelf-life compared to those with refined olive oil.
• The 0.10 mole O3/100 g oil formulation extended the shelf-life of non-preserved emulsions to six months.
• Ozonated oil emulsions exhibited weak antimicrobial effects against Candida albicans and Aspergillus brasiliensis.
• Ozonated olive oil demonstrated enhanced antimicrobial activity against E. coli, S. aureus, C. albicans, and A. brasiliensis, with no cytotoxicity.

Conclusions:

• Ozonated olive oil possesses unique properties that enhance the stability and functionality of cosmetic emulsions.
• The improved shelf-life and antimicrobial activity make ozonated olive oil a promising ingredient for the cosmetics and pharmaceutical industries.
• Further research into specific applications and optimal ozonation parameters is warranted.