Related Experiment Video
Updated: Nov 3, 2025

Production and Analysis of Sporosarcina pasteurii Biocement Bricks Using Custom 3D-Printed Molds for Unconfined Compression Tests
Published on: March 7, 2025
Prolonging Bacterial Viability in Biological Concrete: Coated Expanded Clay Particles
Ronaldas Jakubovskis1, Augusta Jankutė1,2, Simona Guobužaitė1,2
1Institute of Building and Bridge Structures Laboratory of Innovative Building Structures, Vilnius Gediminas Technical University, 10223 Vilnius, Lithuania.
This study explored coated expanded clay (EC) as a bacterial carrier for self-healing concrete. Coated EC improved long-term bacterial viability, leading to enhanced concrete crack healing efficiency.
Area of Science:
- Civil Engineering
- Materials Science
- Microbiology
Background:
- Ensuring long-term bacterial viability in biological self-healing concrete is a significant developmental challenge.
- Bacteria are crucial for the self-healing properties of concrete, but their survival within the harsh concrete matrix is limited.
- Novel carrier materials are needed to protect embedded bacteria and maintain their viability over extended periods.
Purpose of the Study:
- To investigate the efficacy of coated expanded clay (EC) as a carrier for bacteria in biological self-healing concrete.
- To evaluate the long-term viability of bacteria encapsulated within different EC coatings.
- To correlate bacterial viability with the crack healing performance of the developed biological concrete.
Main Methods:
- Eight different coating materials were applied to expanded clay (EC) particles.
- Coated EC particles were incorporated into concrete mixtures as bacterial carriers.
- Long-term bacterial viability was assessed over 56 days.
- Crack closure ratios were measured to evaluate healing efficiency in biological concrete specimens.
Main Results:
- The study found a strong correlation between the number of viable bacteria and the healing efficiency of biological concrete.
- Specimens with higher viable bacteria counts demonstrated superior crack closure.
- Coated expanded clay (EC) particles significantly improved the long-term viability of embedded bacteria.
- The evaluated coating materials varied in their effectiveness in preserving bacterial viability.
Conclusions:
- Coated expanded clay (EC) is a promising carrier material for enhancing long-term bacterial viability in biological self-healing concrete.
- Improved bacterial viability directly translates to more efficient crack healing in concrete.
- The selection of appropriate coating materials is critical for optimizing the performance of biological concrete.
Related Concept Videos
Waterproofing and Anti-Bacterial Admixtures in Concrete
Waterproofing admixtures render concrete hydrophobic,...
Alkali Aggregate Reaction in Concrete
Accelerated Curing of Concrete
Curing of Concrete
Permeability of Concrete
Frost Resistant Concrete
Introducing microscopic air bubbles into the concrete mix through air entrainment creates small voids that accommodate ice expansion, thereby reducing internal pressures and preventing cracking. The optimal amount of...

