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Reprogramming Extracellular Vesicles for Protein Therapeutics Delivery
Leyla A Ovchinnikova1, Stanislav S Terekhov1,2, Rustam H Ziganshin1
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, Russia.
Pharmaceutics
|June 2, 2021
Summary
Engineered extracellular vesicles (EVs) efficiently deliver protein therapeutics using enveloped protein nanocages (EPNs). This system enhances drug delivery into target cells, showing great potential for medical applications.
Area of Science:
- Biotechnology
- Cell Biology
- Nanomedicine
Background:
- Targeted delivery of protein therapeutics is crucial for enhancing drug efficacy.
- Natural membrane-based systems, like extracellular vesicles (EVs), offer biocompatibility and stability for drug delivery.
- Engineered EVs present a promising platform for overcoming delivery challenges.
Purpose of the Study:
- To evaluate engineered, genetically encoded extracellular vesicles (EVs) for efficient protein therapeutic delivery.
- To assess the potential of enveloped protein nanocages (EPNs) for cargo loading within EVs.
- To investigate the therapeutic applications of engineered EVs.
Main Methods:
- Development of a system to encapsulate enveloped protein nanocages (EPNs) within fusogenic EVs via VSV-G overexpression.
- Utilizing bio-safe Fos-Jun heterodimerization for enhanced active cargo loading.
- Proteomic profiling of engineered EVs to assess therapeutic potential.
Main Results:
- Engineered EVs demonstrated efficient cargo loading and delivery into target cells.
- Fos-Jun heterodimerization significantly increased the efficacy of active cargo loading.
- Proteomic analysis provided a comprehensive evaluation of the engineered EVs' therapeutic potential.
Conclusions:
- Engineered EV systems hold significant technological and biomedical potential for protein therapeutic delivery.
- These systems can be applied in areas such as adoptive cell transfer and targeted cell reprogramming.
- The developed platform offers a robust method for enhancing drug delivery efficiency.
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