Non-Enzymatic Glucose Biosensor Based on Highly Pure TiO₂ Nanoparticles

This study proposes a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO₂ nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method. The structural properties of the deposited TiO₂ nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system. The chemical composition and structural properties of the TiO₂ nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM). The glucose detection characteristics of the fabricated biosensor were determined by cyclic voltammetry (CV). CS-PPy/TiO₂ biosensor showed high sensitivity of 302.0 µA mM^-1 cm^-2 (R² = 0.9957) and low detection limit of 6.7 μM. The easily manufactured CS-PPy/TiO₂ biosensor showed excellent selectivity and reactivity.