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Related Experiment Video

Updated: Nov 3, 2025

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Lung on a Chip Development from Off-Stoichiometry Thiol-Ene Polymer.

Roberts Rimsa1, Artis Galvanovskis2, Janis Plume2

  • 1Institute of Solid-State Physics, University of Latvia, 8 Kengaraga Str., LV-1063 Riga, Latvia.

Micromachines
|June 2, 2021
PubMed
Summary

Off-stoichiometry thiol-ene (OSTE) offers improved small molecule absorption for lung on a chip (LOAC) models compared to polydimethylsiloxane (PDMS). However, OSTE’s optical properties currently hinder immunofluorescence analysis in these advanced respiratory research tools.

Keywords:
PDMSlung on a chipoff-stoichiometry thiol–eneorgan on a chip

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Area of Science:

  • Biomaterials Science
  • Respiratory Medicine
  • Microfluidics

Background:

  • Current in vitro lung models face limitations in respiratory disease research and drug repurposing.
  • Lung on a chip (LOAC) technology presents a promising alternative, but polydimethylsiloxane (PDMS) material properties pose challenges.
  • PDMS's absorption of small hydrophobic molecules impedes its use in drug repurposing applications for respiratory diseases.

Purpose of the Study:

  • To evaluate off-stoichiometry thiol-ene (OSTE) as an alternative material for LOAC fabrication.
  • To compare the material properties of OSTE-based LOAC devices against traditional PDMS-based devices.
  • To assess OSTE's suitability for advanced respiratory research and drug development.

Main Methods:

  • Material characterization of OSTE, including light transmission and small molecule absorption.
  • Fabrication of LOAC devices using both OSTE and PDMS.
  • Functionalization of LOAC devices with human umbilical vein endothelial cells (HUVEC) and A549 cell lines.
  • Analysis of cellular responses and immunofluorescence in both OSTE and PDMS LOAC devices.

Main Results:

  • OSTE demonstrated significantly lower small molecule absorption compared to PDMS.
  • OSTE exhibited similar absorption of membrane particles and effects on enzymatic reactions as PDMS.
  • OSTE's lower light transmission negatively impacted the quality of immunofluorescence imaging in LOAC devices.
  • OSTE-based LOACs showed potential for improved drug screening due to reduced molecule absorption.

Conclusions:

  • OSTE is a viable alternative material for developing LOAC devices, offering advantages in reduced small molecule absorption.
  • The optical properties of OSTE require further optimization to overcome limitations in imaging techniques like immunofluorescence.
  • Future OSTE LOAC prototypes should address optical challenges to fully leverage the material's benefits for respiratory research and drug repurposing.