Recent Advancements in Enzyme-Based Lateral Flow Immunoassays.

Area of Science:

• Biomedical Diagnostics
• Analytical Chemistry

Background:

• Paper-based lateral-flow immunoassays (LFIAs) are widely used for qualitative diagnostics.
• Current LFIA technology has limitations in quantitative sensitivity.

Purpose of the Study:

• To review recent advancements in highly sensitive LFIA detection technologies.
• To explore the potential of enzyme-based amplification strategies for improving LFIA performance.

Main Methods:

• Review of literature on enzyme-based amplification strategies in LFIAs.
• Analysis of recent progresses in sensitive tracers and detection methods.
• Adaptation of advanced techniques to portable and miniaturized LFIA formats.

Main Results:

• Enzyme-based amplification strategies offer significant improvements in LFIA sensitivity.
• Integration of advanced tracers and detection instruments enhances quantitative capabilities.
• Portable and miniaturized LFIA formats are enabling ultrasensitive detection.

Conclusions:

• Enzyme-based amplification is a key strategy for transforming LFIAs into ultrasensitive quantitative tools.
• Continued development in LFIA technology promises broader diagnostic applications.
• Future research should focus on overcoming challenges in portable, ultrasensitive LFIA implementation.