Ultrasonic Synthesis and Biomedical Application of Mn_0.5Zn_0.5Er_xY_xFe_2-2xO₄ Nanoparticles

In the present study, biocompatible manganese nanoparticles have been linked with zinc and iron molecules to prepare different derivatives of Mn_0.5Zn_0.5Er_xY_xFe_2-2xO₄ NPs (x = 0.02, 0.04, 0.06, 0.08, 0.10), using an ultrasonication approach. The structure, surface morphology, and chemical compositions of Mn_0.5Zn_0.5Er_xY_xFe_2-2xO₄ NPs were elucidated by X-ray diffractometer (XRD), High-resolution transmission electron microscopy (HR-TEM), scanning electron microscope (SEM), and Energy Dispersive X-Ray Analysis (EDX) techniques. The bioactivity of Mn_0.5Zn_0.5Er_xY_xFe_2-2xO₄ NPs on normal (HEK-293) and (HCT-116) colon cancer cell line was evaluated. The Mn_0.5Zn_0.5Er_xY_xFe_2-2xO₄ NPs treatment post 48 h resulted in a significant reduction in cells (via MTT assay, having an IC₅₀ value between 0.88 µg/mL and 2.40 µg/mL). The specificity of Mn_0.5Zn_0.5Er_xY_xFe_2-2xO₄ NPs were studied by treating them on normal cells line (HEK-293). The results showed that Mn_0.5Zn_0.5Er_xY_xFe_2-2xO₄ NPs did not incur any effect on HEK-293, which suggests that Mn_0.5Zn_0.5Er_xY_xFe_2-2xO₄ NPs selectively targeted the colon cancerous cells. Using Candida albicans, antifungal activity was also studied by evaluating minimum inhibitory/fungicidal concentration (MIC/MFC) and the effect of nanomaterial on the germ tube formation, which exhibited that NPs significantly inhibited the growth and germ tube formation. The obtained results hold the potential to design nanoparticles that lead to efficient bioactivity.