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Published on: March 10, 2016
Effective Microorganisms (EM) Improve Internal Organ Morphology, Intestinal Morphometry and Serum Biochemical
Korina Michalska1, Michał Gesek1, Rajmund Sokół2
1Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 13, 10-719 Olsztyn, Poland.
Abstract:
The effect of effective microorganisms (EM) on internal organ morphology, intestinal morphometry, and serum biochemical activity in Japanese quails under Clostridium perfringens challenge was determined. After 30 days of EM addition, one group of quails was orally inoculated with Clostridium perfringens. The second group did not receive EM and was inoculated with C. perfringens. In the gut, EM supplementation reduced the number of lesions, enhanced gut health, and protected the mucosa from pathogenic bacteria. EM showed an anti-inflammatory effect and fewer necrotic lesions in villi. In the internal organs, EM showed a protective effect against a typical lesion of C. perfringens infection. Necrosis and degeneration of the hepatocytes, necrosis of bile ducts, and bile duct proliferation were more severe in the infected group without EM. Morphometric evaluation showed significantly higher villi in the jejunum after EM addition. A greater crypt depth was observed in the C. perfringens group. Biochemical analysis of the blood indicated lower cholesterol on the 12th day of the experiment and between-group differences in total protein, lactate dehydrogenase (LDH), and albumin levels in the EM group. Further studies are needed to improve EM activity against pathologic bacteria as a potential alternative to antibiotics and to develop future natural production systems.
Insights
Effective microorganisms (EM) improved gut health and protected Japanese quails from Clostridium perfringens infection. EM supplementation reduced lesions and enhanced intestinal morphology, showing potential as a natural alternative to antibiotics.
Area of Science:
- Animal Science
- Microbiology
- Veterinary Medicine
Background:
- Clostridium perfringens poses a significant threat to poultry health, causing intestinal damage and economic losses.
- Effective Microorganisms (EM) are a consortium of beneficial microbes with potential applications in animal health and disease prevention.
Purpose of the Study:
- To evaluate the impact of EM supplementation on Japanese quails challenged with Clostridium perfringens.
- To assess EM's effects on internal organ morphology, intestinal morphometry, and serum biochemistry.
Main Methods:
- Japanese quails were supplemented with EM for 30 days.
- Groups were challenged orally with Clostridium perfringens.
- Internal organs, intestinal morphology, and serum biochemical parameters were analyzed.
Main Results:
- EM supplementation reduced intestinal lesions and protected gut mucosa from pathogenic bacteria.
- EM exhibited anti-inflammatory effects and fewer necrotic lesions in villi.
- EM protected internal organs from C. perfringens-induced damage, with improved jejunal villi height and altered biochemical profiles.
Conclusions:
- EM supplementation demonstrates a protective effect against Clostridium perfringens challenge in Japanese quails.
- EM enhances gut health, improves intestinal morphology, and modulates serum biochemistry.
- Further research is warranted to optimize EM efficacy as a natural alternative to antibiotics in poultry production.
