Patterns in Genotype Composition of Indian Isolates of the Bombyx mori Nucleopolyhedrovirus and Bombyx mori

Related Experiment Video

Updated: Nov 3, 2025

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
06:25

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Published on: February 8, 2020

5.9K

Mudasir Gani1, Sergei Senger2, Satish Lokanath3

  • 1Division of Entomology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences & Technology, Kashmir 193 201, J&K, India.

Viruses
|June 2, 2021
PubMed

Insights

Indian mulberry silkworm viruses, Bombyx mori nucleopolyhedrovirus (BmNPV) and Bombyx mori bidensovirus (BmBDV), show genetic diversity. Analysis reveals mixed viral populations and distinct genetic compositions in different regions, impacting sericulture.

Area of Science:

  • * Virology
  • * Genomics
  • * Entomology

Background:

  • * The mulberry silkworm (Bombyx mori) is economically significant.
  • * Viral diseases caused by Bombyx mori nucleopolyhedrovirus (BmNPV) and Bombyx mori bidensovirus (BmBDV) cause substantial losses in the sericulture industry.
  • * Understanding the genetic makeup of Indian viral isolates is crucial for disease management.

Purpose of the Study:

  • * To analyze the population structure and genetic composition of Indian BmNPV isolates.
  • * To investigate the genetic variability within and between different geographic isolates of BmNPV.
  • * To identify the presence and genetic characteristics of co-occurring BmBDV in Indian silkworm samples.

Main Methods:

  • * Phylogenetic analysis of partial polh, lef-8, and lef-9 gene fragments from seven new Indian BmNPV isolates.
  • * Whole-genome sequencing of three selected Indian BmNPV isolates (Mysore, Jammu, Dehradun).
  • * Analysis of single nucleotide polymorphism (SNP) positions and nucleotide frequencies to assess intra-isolate genetic variability.

Main Results:

  • * Seven new Indian BmNPV isolates were identified and grouped within the BmNPV clade, showing close relation to AcMNPV and RoMNPV.
  • * BmNPV isolates from Dehradun (BmNPV-De) and Jammu (BmNPV-Ja) exhibited high genotypic similarity and contained significant amounts of BmBDV.
  • * The BmNPV isolate from Mysore (BmNPV-My) presented a pure and homogenous population structure with minimal genetic variation.

Conclusions:

  • * Indian BmNPV isolates exhibit genetic diversity, with distinct population structures observed across different regions.
  • * Co-infection with BmBDV is prevalent in certain Indian BmNPV isolates, particularly in Dehradun and Jammu.
  • * The findings enhance understanding of the genetic diversity, evolution, and complex interactions of BmNPV and BmBDV in India, with implications for sericulture disease control.
Keywords:
BaculoviridaeBidnaviridaeBombyx moribaculovirusdensovirusgenetic variationgenotypepopulation structuresilkwormsingle nucleotide polymorphism

More Related Videos

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes06:41

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Published on: August 31, 2022

2.2K
Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm11:12

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

Published on: April 12, 2017

8.7K

Related Concept Videos

Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
312
Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
24.2K
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
284