Synthesis, Characterization, and Electrospinning of a Functionalizable, Polycaprolactone-Based Polyurethane for Soft...

Area of Science:

• Biomaterials Science
• Polymer Chemistry
• Tissue Engineering

Background:

• Developing advanced scaffolds is crucial for soft tissue engineering.
• Biodegradable and functionalizable materials are needed for effective tissue regeneration.
• Polyurethanes offer tunable properties for biomedical applications.

Purpose of the Study:

• To synthesize and characterize a biodegradable, elastomeric, and functionalizable polyurethane (PU-DMPA).
• To evaluate its potential as an electrospun scaffold for soft tissue engineering.
• To demonstrate the functionalization capabilities of the synthesized PU.

Main Methods:

• Two-step polymerization to synthesize PU-DMPA and a control PU-BDO.
• Characterization using FT-IR, 1H-NMR, and thermal analysis.
• Mechanical testing, hydrophilicity assessment, and cell adhesion studies.
• Electrospinning to fabricate membranes and functionalization via carbodiimide chemistry.

Main Results:

• PU-DMPA exhibited desired elastomeric properties and improved hydrophilicity compared to PU-BDO.
• Successful fabrication of bead-free electrospun PU-DMPA membranes.
• Enhanced adhesion of smooth muscle cells on PU-DMPA.
• Demonstrated functionalization with gelatin and hyaluronic acid.

Conclusions:

• PU-DMPA is a promising biodegradable and functionalizable material for soft tissue engineering scaffolds.
• Its elastomeric properties and ability to be electrospun are advantageous.
• The demonstrated functionalization opens avenues for tailored tissue regeneration strategies.