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Updated: Nov 3, 2025

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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The Lysine Methylase SMYD3 Modulates Mesendodermal Commitment during Development

Raffaella Fittipaldi1, Pamela Floris1, Valentina Proserpio1,2

  • 1Department of Biosciences, University of Milan, Via Celoria 26, 20133 Milan, Italy.

Cells
|June 2, 2021
PubMed

Insights

SET and MYND domain containing protein 3 (SMYD3) regulates mesendodermal markers during early development. This study reveals SMYD3

Area of Science:

  • Developmental Biology
  • Epigenetics
  • Cancer Biology

Background:

  • SET and MYND domain containing protein 3 (SMYD3) is a methylase implicated in oncogenesis and overexpressed in cancer.
  • Its role in normal physiological development remains largely uncharacterized.

Purpose of the Study:

  • To investigate the function of SMYD3 during early embryonic development.
  • To elucidate the role of SMYD3 in embryonic stem cell differentiation and zebrafish development.

Main Methods:

  • Utilized mouse embryonic stem cells (ESCs) for in vitro differentiation studies.
  • Employed zebrafish as an in vivo model system for genetic knockdown experiments.

Main Results:

  • SMYD3 depletion in ESCs promoted mesodermal pattern induction and upregulated cardiovascular lineage markers.
  • Knockdown of SMYD3 in zebrafish led to increased mesendodermal marker expression during gastrulation.

Conclusions:

  • SMYD3 plays a crucial role in modulating mesendodermal marker levels during early development.
  • These findings highlight a novel function for SMYD3 in developmental processes beyond its known oncogenic roles.
Keywords:
SMYD3developmentembryonic stem cellszebrafish

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