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Updated: Nov 3, 2025
Electric and Magnetic Field Devices for Stimulation of Biological Tissues
Published on: May 15, 2021
Modulation of Cellular Response to Different Parameters of the Rotating Magnetic Field (RMF)-An In Vitro Wound
Magdalena Jedrzejczak-Silicka1, Marian Kordas2, Maciej Konopacki2
1Laboratory of Cytogenetics, West Pomeranian University of Technology in Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland.
Abstract:
Since the effect of MFs (magnetic fields) on various biological systems has been studied, different results have been obtained from an insignificant effect of weak MFs on the disruption of the circadian clock system. On the other hand, magnetic fields, electromagnetic fields, or electric fields are used in medicine. The presented study was conducted to determine whether a low-frequency RMF (rotating magnetic field) with different field parameters could evoke the cellular response in vitro and is possible to modulate the cellular response. The cellular metabolic activity, ROS and Ca2+ concentration levels, wound healing assay, and gene expression analyses were conducted to evaluate the effect of RMF. It was shown that different values of magnetic induction (B) and frequency (f) of RMF evoke a different response of cells, e.g., increase in the general metabolic activity may be associated with the increasing of ROS levels. The lower intracellular Ca2+ concentration (for 50 Hz) evoked the inability of cells to wound closure. It can be stated that the subtle balance in the ROS level is crucial in the wound for the effective healing process, and it is possible to modulate the cellular response to the RMF in the context of an in vitro wound healing.
Insights
Rotating magnetic fields (RMF) can modulate cellular responses, impacting metabolic activity and wound healing. Specific RMF parameters influence cell behavior, highlighting the potential for targeted biological effects.
Area of Science:
- Biophysics
- Cell Biology
- Biomedical Engineering
Background:
- Magnetic fields (MFs) have shown varied effects on biological systems, with some studies indicating minimal impact on circadian rhythms.
- Magnetic, electromagnetic, and electric fields are established tools in medical applications.
- Investigating low-frequency rotating magnetic fields (RMF) offers potential for novel therapeutic interventions.
Purpose of the Study:
- To determine if low-frequency RMF can elicit cellular responses in vitro.
- To explore the possibility of modulating cellular responses using RMF with varying parameters.
- To assess the impact of RMF on cellular metabolic activity, reactive oxygen species (ROS), intracellular calcium (Ca2+), and gene expression.
Main Methods:
- In vitro cell culture models.
- Exposure to low-frequency rotating magnetic fields (RMF) with varied magnetic induction (B) and frequency (f).
- Assays for cellular metabolic activity, ROS levels, intracellular Ca2+ concentration, wound healing, and gene expression analysis.
Main Results:
- Different RMF parameters (B and f) induced distinct cellular responses.
- Increased metabolic activity correlated with elevated ROS levels.
- Lower intracellular Ca2+ concentration at 50 Hz impaired cellular wound closure.
- RMF can modulate cellular behavior in an in vitro wound healing context.
Conclusions:
- The cellular response to RMF is dependent on specific field parameters.
- Maintaining a balanced ROS level is critical for effective wound healing.
- RMF presents a viable method for modulating cellular responses, particularly in in vitro wound healing models.
