Related Experiment Video
Updated: Nov 3, 2025
06:02
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
Published on: September 13, 2018
7.1K
Applications of Deep Mutational Scanning in Virology
Thomas D Burton1, Nicholas S Eyre1
1College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, Australia.
Viruses
|June 2, 2021
Summary
Deep mutational scanning is a powerful protein science technique for analyzing viral genomes. This review covers its application in RNA viruses like SARS-CoV-2 and influenza, aiding in understanding viral evolution.
Area of Science:
- Molecular virology
- Protein science
- Genomics
Background:
- High-throughput techniques are revolutionizing molecular virology.
- Methods like proteomics, CRISPR screens, and ChIP-seq offer new insights into viral infections.
- Understanding host-virus interactions and viral evolution is crucial.
Purpose of the Study:
- To review the application of deep mutational scanning (DMS) in RNA virus research.
- To highlight DMS's utility in functional analysis of viral genes and genomes.
- To discuss related technologies like reverse genetics and next-generation sequencing.
Main Methods:
- Deep mutational scanning (DMS) for in-depth nucleotide-level functional analysis.
- Application of DMS to RNA viruses, including Flaviviridae, Influenza A Virus, and SARS-CoV-2.
- Integration with reverse genetics, next-generation sequencing, and bioinformatics tools.
Main Results:
- DMS reveals critical regions, flexibility, and mutational potential within viral genomes.
- Provides a comprehensive understanding of viral protein function and evolution.
- Facilitates the identification of host factors involved in viral replication and pathogenesis.
Conclusions:
- Deep mutational scanning is a key technique for advancing RNA virus research.
- Its application aids in understanding viral adaptation, drug resistance, and vaccine development.
- The integration of DMS with other high-throughput methods offers powerful insights into virology.
Related Concept Videos
00:36Viral Mutations
36.5K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
36.5K
01:18Mutations in Microorganisms
221
Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
221
02:28Leaky Scanning
5.3K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.3K