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An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells
Published on: December 16, 2019
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Novel Methods to Mobilize, Isolate, and Expand Mesenchymal Stem Cells
Cristiano P Vieira1, Taralyn M McCarrel2, Maria B Grant1
1Department of Ophthalmology and Visual Sciences, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
International Journal of Molecular Sciences
|June 2, 2021
Summary
Two novel methods efficiently produce mesenchymal stem cells (MSCs) from horses and humans. These MSCs show therapeutic potential for metabolic and inflammatory diseases, offering a promising avenue for regenerative medicine.
Area of Science:
- Regenerative Medicine
- Immunology
- Cell Biology
Background:
- Mesenchymal stem cells (MSCs) are crucial for treating metabolic and inflammatory diseases due to their immune-modulating properties.
- Efficient and scalable MSC production is vital for their therapeutic application.
Purpose of the Study:
- To present two novel, efficient methods for obtaining mesenchymal stem cells (MSCs) from equine and human sources.
- To characterize the phenotype and differentiation potential of the derived MSCs.
Main Methods:
- Equine MSCs were mobilized using electro-acupuncture and collected/expanded using the automated CliniMACS Prodigy system.
- Human induced pluripotent stem cells (iPSCs) were differentiated into MSCs via embryoid bodies and FACS sorted.
Main Results:
- The equine method yielded approximately 100 MSC colonies within seven days, expressing key MSC markers (CD105, CD90, CD73).
- Human iPSC-derived MSCs also exhibited the characteristic MSC surface marker profile (CD105, CD90, CD73).
- Both equine and human MSCs demonstrated multipotency, differentiating into adipogenic, osteogenic, and chondrogenic lineages.
Conclusions:
- The described methods offer simple and highly efficient ways to produce MSCs with a confirmed phenotype.
- These approaches hold significant potential for the rapid, large-scale production of MSCs for therapeutic applications in regenerative medicine.
