Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

780
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
780
CRISPR01:59

CRISPR

54.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
54.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.0K
Homologous Recombination02:31

Homologous Recombination

57.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
57.8K
What is Genetic Engineering?00:49

What is Genetic Engineering?

76.5K
Overview
76.5K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.3K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ecological Microenvironment Response of Rhizosphere Soil Microbial Communities to Varying Soil Amendments: Insights from Diversity, Stability, and Multi-Functionality.

Plants (Basel, Switzerland)·2026
Same author

Next-Generation Cyanobacterial Biocontrol Agents for Crop Fungal Diseases: Molecular Mechanisms, Omics Insights, Applications, and Biosafety Considerations.

Journal of basic microbiology·2026
Same author

Magnetic iron oxide nanoparticles modulate photosynthetic energy partitioning and photoprotective dissipation in radish under UV-B stress.

Functional plant biology : FPB·2026
Same author

Transforming soil decontamination: the role and prospects of nanotechnological remediation.

RSC advances·2026
Same author

Agmatine and N-hydroxypipecolic acid synergistically enhance salt tolerance in wheat through antioxidant defense, proline metabolism, and photosynthetic protection.

Plant signaling & behavior·2026
Same author

Buckwheat: A Sustainable Alternative Crop Under Changing Climate.

Journal of visualized experiments : JoVE·2026

Related Experiment Video

Updated: Nov 3, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.4K

CRISPR-Based Genome Editing Tools: Insights into Technological Breakthroughs and Future Challenges.

Muntazir Mushtaq1, Aejaz Ahmad Dar1, Milan Skalicky2

  • 1School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu 180009, India.

Genes
|June 2, 2021
PubMed
Summary

Genome-editing (GE) advances, particularly CRISPR technology, offer sustainable solutions for global food security through crop improvement. This review explores innovations like transgene-free genetically modified organisms (GMOs) and addresses challenges in delivery, precision, and public acceptance.

Keywords:
CRISPRDNA-free genome editingbase editingcrop improvementgenome editingprime editing

More Related Videos

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.7K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

4.7K

Related Experiment Videos

Last Updated: Nov 3, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.4K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.7K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

4.7K

Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Biotechnology

Background:

  • Genome-editing (GE) is revolutionizing life sciences with significant applications.
  • Transgene-free genetically modified organism (GMO) development is a key area of interest.
  • GE technologies hold promise for achieving global food security sustainably.

Purpose of the Study:

  • To review recent developments in CRISPR-based genome-editing tools for crop improvement.
  • To discuss opportunities and challenges associated with these technologies.
  • To provide insights into technological advances, applications, and future hurdles in plant genome editing.

Main Methods:

  • Review of recent advancements in CRISPR-based genome-editing tools.
  • Discussion of innovations including transgene-free genome plants, compatible nucleases, and delivery vehicles.
  • Exploration of multi-gene targeting, base editing, prime editing, and gene regulation fine-tuning.

Main Results:

  • Development of transgene-free genome plants and effective CRISPR delivery systems.
  • Advancements in multi-gene targeting, base editing, and prime editing for complex genetic engineering.
  • Identification of new methods for fine-tuning plant gene regulation.

Conclusions:

  • CRISPR and other GE tools offer tremendous potential for crop improvement and sustainable agriculture.
  • Significant challenges remain in efficient reagent delivery, precision editing, government policies, and public acceptance.
  • Further research and development are crucial for overcoming these challenges and realizing the full potential of GE in agriculture.