Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combined PET-CT and VATS biopsy in pleural effusion: diagnostic accuracy, surgical factors, and prognosis-a retrospective study.

General thoracic and cardiovascular surgery·2026
Same author

Fibroblast activation protein inhibitor-PET/CT in lung cancer: a prospective single-center study with histopathological correlation of fibroblast activation protein expression.

International journal of clinical oncology·2026
Same author

Rhabdomyolysis caused by neuroleptic malignant syndrome requiring long-term hemodialysis: A case report and literature review.

Internal medicine (Tokyo, Japan)·2026
Same author

Distinct fibrosis-associated macrophage subsets coordinate iron metabolism in pulmonary fibrosis.

International immunology·2026
Same author

Elucidating genetic backgrounds of myasthenia gravis in Japanese by genome-wide association studies and multi-omics analyses of thymoma.

Nature communications·2026
Same author

Exploring fibroblast activation protein as an early biomarker in chronic lung allograft dysfunction.

The European respiratory journal·2025

Related Experiment Video

Updated: Nov 3, 2025

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall
11:26

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall

Published on: July 31, 2015

12.7K

Three-Dimensional Idiopathic Pulmonary Fibrosis Model Using a Layer-by-Layer Cell Coating Technique.

Hidenori Kuno1, Takami Akagi2, Eriko Fukui1

  • 1Department of General Thoracic Surgery, Graduate School of Medicine, Osaka University, Suita, Japan.

Tissue Engineering. Part C, Methods
|June 2, 2021
PubMed
Summary

Researchers developed a novel 3D cell model for idiopathic pulmonary fibrosis (IPF) using a layer-by-layer coating technique. This advanced 3D model accurately mimics IPF tissue, aiding antifibrosis drug development.

Keywords:
cell coating techniquedrug screeningidiopathic pulmonary fibrosisin vitro 3D IPF modellayer-by-layer

More Related Videos

Refined Murine Model of Idiopathic Pulmonary Fibrosis
07:51

Refined Murine Model of Idiopathic Pulmonary Fibrosis

Published on: June 17, 2025

497
Generation of Human 3D Lung Tissue Cultures 3D-LTCs for Disease Modeling
05:47

Generation of Human 3D Lung Tissue Cultures 3D-LTCs for Disease Modeling

Published on: February 12, 2019

21.4K

Related Experiment Videos

Last Updated: Nov 3, 2025

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall
11:26

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall

Published on: July 31, 2015

12.7K
Refined Murine Model of Idiopathic Pulmonary Fibrosis
07:51

Refined Murine Model of Idiopathic Pulmonary Fibrosis

Published on: June 17, 2025

497
Generation of Human 3D Lung Tissue Cultures 3D-LTCs for Disease Modeling
05:47

Generation of Human 3D Lung Tissue Cultures 3D-LTCs for Disease Modeling

Published on: February 12, 2019

21.4K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Pulmonary Medicine

Background:

  • Idiopathic pulmonary fibrosis (IPF) is a severe lung disease marked by fibroblast proliferation and abnormal blood vessel changes.
  • Current limitations exist in in vitro models for replicating IPF's specific cellular environment.
  • Three-dimensional (3D) cell cultures offer a promising avenue for mimicking complex tissue functions and advancing antifibrosis drug discovery.

Purpose of the Study:

  • To establish and evaluate a novel in vitro 3D model for idiopathic pulmonary fibrosis (IPF).
  • To utilize a layer-by-layer (LbL) cell coating technique for constructing a vascularized 3D IPF model.
  • To assess the model's ability to replicate IPF-specific fibroblast function and microvascular abnormalities.

Main Methods:

  • Fabrication of an in vitro 3D IPF model using human lung fibroblasts and microvascular endothelial cells.
  • Application of a layer-by-layer (LbL) cell coating technique to build 3D tissue constructs.
  • Evaluation of the model's drug responsiveness to approved antifibrosis medications.

Main Results:

  • The LbL technique successfully created a 3D IPF model that mimics fibroblast-specific functions and aberrant microvascular structures.
  • The developed in vitro IPF-3D model demonstrated responsiveness to two globally approved antifibrosis drugs.
  • The model provides a platform for visualizing fibroblast behavior and the pulmonary microvascular environment in IPF.

Conclusions:

  • The LbL cell coating technique is effective for creating a relevant in vitro IPF-3D model.
  • This novel 3D model can enhance understanding of IPF pathogenesis, including fibroblast activity and microvascular changes.
  • The model shows potential for predicting the efficacy of new antifibrosis therapies, accelerating drug development.