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Related Experiment Videos

Structural insights into dpCoA-RNA decapping by NudC.

Wei Zhou1, Zeyuan Guan1, Fen Zhao1

  • 1National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.

RNA Biology
|June 2, 2021
PubMed
Summary
This summary is machine-generated.

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Escherichia coli NudC protein hydrolyzes the dpCoA cap on RNA, similar to NAD cap removal. This enzyme and its homologs in yeast and plants show conserved dpCoA decapping activity, revealing a common RNA cap hydrolysis mechanism.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • RNA 5' cap structures like m7G, triphosphate, NAD, and dpCoA protect RNA termini.
  • These caps influence RNA stability, translation, and transport.
  • Nudix hydrolase family proteins, including NudC, are known to remove various RNA caps.

Purpose of the Study:

  • To investigate the hydrolytic activity of Escherichia coli NudC on the dpCoA RNA cap.
  • To determine the crystal structure of NudC in complex with dpCoA.
  • To explore the conserved mechanism of dpCoA-capped RNA hydrolysis in prokaryotes and eukaryotes.

Main Methods:

  • In vitro biochemical assays to assess NudC's hydrolytic activity on dpCoA and dpCoA-capped RNA.
  • X-ray crystallography to determine the 2.0 Å resolution crystal structure of dimeric NudC with dpCoA.
Keywords:
Cap structuresNAD capNudCadenine dinucleotide derivative capsdpCoA decappingm7G

Related Experiment Videos

  • Functional analysis of NudC homologs in Saccharomyces cerevisiae and Arabidopsis thaliana.

    • Main Results:

    • Escherichia coli NudC demonstrates hydrolytic activity against dpCoA and dpCoA-capped RNA in vitro.
    • The crystal structure reveals dpCoA recognition and hydrolysis mechanisms similar to NAD.
    • NudC homologs in yeast and plants also exhibit dpCoA decapping (deCoAping) activity.

    Conclusions:

    • NudC hydrolyzes the dpCoA RNA cap through a mechanism analogous to NAD cap removal.
    • NudC may also process other dinucleotide derivative RNA caps containing AMP moieties.
    • A conserved mechanism for dpCoA-capped RNA hydrolysis exists across prokaryotes and eukaryotes, mediated by NudC and its homologs.