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A novel copper (II) binding peptide for a colorimetric biosensor system design.

Nuriye Korkmaz1, Changhyun Hwang2, Kim Kristin Kessler1

  • 1Biosensor Group, Korea Institute of Science and Technology Europe Forschungsgesellschaft MbH, Campus E 7.1, D-66123, Saarbrücken, Germany.

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|June 2, 2021
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Summary

Researchers developed a novel biosensor using engineered bacteriophages to detect copper ions (Cu(II)). This cost-effective method offers high selectivity and sensitivity for environmental monitoring and water quality assessment.

Keywords:
BiosensorCopperFilamentous phagesGenetic engineeringPhage display

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Area of Science:

  • Biotechnology
  • Environmental Science
  • Materials Science

Background:

  • Filamentous bacteriophages infect bacteria and can be engineered for specific applications.
  • Copper(II) (Cu(II)) is a significant environmental pollutant requiring sensitive detection methods.

Purpose of the Study:

  • To identify highly selective Cu(II) binding peptides using phage display.
  • To develop a cost-effective and user-friendly biosensor for Cu(II) detection.

Main Methods:

  • Phage display technique for peptide selection against Cu(II) and competitive metal ions.
  • Enzyme Linked Immunosorbent Assay (ELISA), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray spectroscopy (EDX) for confirmation.
  • Development of a gold nanoparticle (AuNP)-peptide biosensor for Cu(II) detection.

Main Results:

  • Identified Cu-5 peptide (HGFANVA) with high Cu(II) affinity.
  • Engineered viruses demonstrated strong Cu(II) binding potential confirmed by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
  • AuNP-based biosensor showed high selectivity for Cu(II) with a low limit of detection (LOD) of 91.15 nM, well below WHO guidelines.

Conclusions:

  • Short peptides derived from bacteriophages can serve as effective recognition units for biosensor development.
  • The developed AuNP-peptide biosensor is selective, sensitive, user-friendly, and cost-effective for Cu(II) detection.