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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

12.2K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Related Experiment Video

Updated: Nov 3, 2025

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
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Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

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Detecting Fluorochrome-Labeled Cells.

Scott J Rodig

    Cold Spring Harbor Protocols
    |June 2, 2021
    PubMed
    Summary
    This summary is machine-generated.

    This protocol details cell staining with fluorochrome-labeled antibodies, enhancing subcellular structure resolution beyond light microscopy. Advances in fluorochromes offer brighter, longer-lasting dyes for improved immunofluorescence imaging.

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    Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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    Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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    Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

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    Area of Science:

    • Immunology
    • Cell Biology
    • Microscopy

    Background:

    • Cell staining protocols are crucial for visualizing subcellular structures.
    • Fluorochrome-labeled antibodies offer superior resolution compared to traditional light microscopy.
    • Advances in fluorochrome technology have led to brighter and more stable dyes.

    Purpose of the Study:

    • To describe a protocol for cell staining using fluorochrome-labeled antibodies.
    • To highlight the advantages of fluorochrome-based imaging for subcellular resolution.
    • To discuss recent advancements in fluorochrome development and availability.

    Main Methods:

    • Utilizing fluorochrome-labeled antibodies for cell staining.
    • Employing advanced fluorochromes like fluorescein, rhodamine, and Texas Red.
    • Using specific filter sets for independent observation of multiple fluorochromes.

    Main Results:

    • Fluorochrome-labeled antibodies provide enhanced resolution of subcellular details.
    • Newer fluorochromes offer improved brightness and photostability.
    • Commercial availability of conjugates and filter sets simplifies the protocol.

    Conclusions:

    • Cell staining with fluorochrome-labeled antibodies is a powerful technique for high-resolution imaging.
    • Ongoing developments in fluorochromes continue to enhance immunofluorescence applications.
    • The described protocol leverages modern fluorochrome technology for effective cell visualization.