Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aircraft observations of black carbon over the Yellow Sea and Seoul Metropolitan Area: Vertical profiles and air mass origin influence.

Journal of environmental sciences (China)·2026
Same author

Acoustofluidic separation of oblate spheroids from spheres using acoustic radiation torque and force.

Lab on a chip·2026
Same author

Elasto-Inertial Microfluidic Separation of Prolate Ellipsoids and Spheroids in a Coflow of Newtonian and Viscoelastic Fluids.

Analytical chemistry·2026
Same author

Elasto-Inertial Microfluidics for Particle Manipulation Using Co-flow of Newtonian and Viscoelastic Fluids.

Analytical chemistry·2026
Same author

The 2026 guided acoustic waves roadmap.

Journal of physics D: Applied physics·2026
Same author

Microfluidic shape-based separation for cells and particles: recent progress and future perspective.

Lab on a chip·2026

Related Experiment Video

Updated: Nov 3, 2025

Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads
06:08

Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

Published on: October 17, 2022

2.7K

Acoustofluidic Separation of Proteins Using Aptamer-Functionalized Microparticles.

Muhammad Afzal1, Jinsoo Park2, Jessie S Jeon1

  • 1Department of Mechanical Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea.

Analytical Chemistry
|June 2, 2021
PubMed
Summary

This study introduces a novel acoustofluidic method for separating three types of human proteins simultaneously. The technique utilizes traveling surface acoustic waves (TSAWs) to separate protein-conjugated microparticles based on size, enabling efficient triseparation.

More Related Videos

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

14.0K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.3K

Related Experiment Videos

Last Updated: Nov 3, 2025

Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads
06:08

Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

Published on: October 17, 2022

2.7K
A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

14.0K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.3K

Area of Science:

  • Biotechnology
  • Microfluidics
  • Acoustic Physics

Background:

  • Protein separation is crucial for diagnostics and research.
  • Existing methods face challenges in efficiency and multiplexing.
  • Acoustofluidics offers a label-free approach for particle manipulation.

Purpose of the Study:

  • To develop and demonstrate an acoustofluidic method for simultaneous triseparation of proteins.
  • To utilize traveling surface acoustic waves (TSAWs) for size-dependent microparticle manipulation.
  • To achieve multiplexed protein capture and separation using aptamer-functionalized microparticles.

Main Methods:

  • Fabrication of an acoustofluidic device with a slanted-finger interdigital transducer (SFIT) on a lithium niobate substrate.
  • Integration of a polydimethylsiloxane (PDMS) microfluidic channel for sample handling.
  • Application of TSAWs to generate acoustic radiation force (ARF) for separating microparticles of different sizes.
  • Functionalization of microparticles with aptamers for specific protein capture (thrombin and immunoglobulin E).

Main Results:

  • Successful demonstration of simultaneous triseparation of three human proteins: thrombin, immunoglobulin E, and mCardinal2.
  • Separation achieved by exploiting size-dependent acoustic radiation force (ARF) acting on aptamer-conjugated microparticles.
  • High throughput and label-free separation of target proteins within the microfluidic channel.

Conclusions:

  • The proposed acoustofluidic method enables efficient simultaneous triseparation of proteins.
  • TSAWs provide a powerful, label-free mechanism for size-based separation of biomolecules.
  • This technology holds promise for advanced diagnostics and biochemical analysis.