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Updated: Nov 3, 2025
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Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution
Published on: May 2, 2020
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Double network laminarin-boronic/alginate dynamic bioink for 3D bioprinting cell-laden constructs
Adérito J R Amaral1, Vítor M Gaspar1, Pedro Lavrador1
1CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Biofabrication
|June 2, 2021
Summary
Researchers developed a dynamic hydrogel bioink using laminarin and alginate for 3D bioprinting. This bioink supports cell growth and enables the creation of customizable, mechanically tunable tissue engineering scaffolds.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Bioprinting
Background:
- Dynamically crosslinked hydrogel bioinks are crucial for fabricating mechanically tuneable, cell-laden constructs for 3D *in vitro* disease modelling and tissue engineering.
- Developing advanced bioinks is essential for creating sophisticated 3D *in vitro* models and regenerative medicine applications.
Purpose of the Study:
- To explore a dynamic bioink composed of boronic acid-functionalised laminarin and alginate for 3D bioprinting.
- To create mechanically tuneable, cell-laden 3D constructs with user-programmable architecture.
Main Methods:
- Formulation of a dynamic bioink using boronic acid-functionalised laminarin and alginate.
- Utilisation of a double crosslinked network combining reversible boronate ester bonds and ionic gelation.
- Bioprinting of 3D constructs and assessment of rheological properties, mechanical features, and cell viability.
Main Results:
- The bioink exhibited suitable rheological properties and enhanced mechanical features due to its modular crosslinking chemistry.
- Stable 3D constructs with user-programmable architecture were successfully bioprinted.
- High cell viability (>90%) and homogeneous cell distribution were observed for osteoblast precursors, fibroblasts, and breast cancer cells over 14 days in culture.
Conclusions:
- This dynamic bioink offers a simple and versatile approach for fabricating biomimetic 3D scaffolds.
- The developed bioink has broad applicability in predictive or exploratory biomedical platforms, accelerating the development of multifunctional bioinks.
