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Related Experiment Videos

Biotinidase in human breast milk.

J Oizumi1, K Hayakawa

  • 1National Children's Medical Research Center, Division of Metabolism, Tokyo, Japan.

The American Journal of Clinical Nutrition
|August 1, 1988
PubMed
Summary

Human breast milk contains biotinidase activity, crucial for infant nutrition. Colostrum shows significantly higher activity than mature milk, highlighting its importance.

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Area of Science:

  • Biochemistry
  • Human Physiology
  • Nutritional Science

Background:

  • Biotinidase is an enzyme essential for biotin metabolism.
  • Biotinidase activity in human breast milk has not been extensively characterized.
  • Understanding biotinidase levels in milk is important for infant nutrition.

Purpose of the Study:

  • To quantify biotinidase activity in human breast milk.
  • To compare biotinidase activity in colostrum versus mature milk.
  • To assess the nutritional significance of biotinidase in breast milk.

Main Methods:

  • Development of a sensitive high-performance liquid chromatography (HPLC)-fluorometric assay.
  • Analysis of 19 human breast milk samples.
  • Comparison of milk biotinidase activity with human serum values.

Main Results:

  • All tested breast milk samples demonstrated detectable biotinidase activity.
  • Mature milk exhibited mean activity of 0.208 nmol/min/mL, with specific activity of 7.51 pmol/min/mg protein.
  • Colostrum displayed approximately five times higher biotinidase activity compared to mature milk.
  • Breast milk biotinidase activity was considerably lower than that found in human serum.

Conclusions:

  • Biotinidase is present in all human breast milk samples analyzed.
  • The enzyme's presence suggests a vital nutritional role for infants.
  • Colostrum's higher activity indicates a potentially greater importance in early neonatal nutrition.

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