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Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
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β-Difluoroalkylamine as a Motif for Singlet Oxygen-Mediated Proximity Labeling in Living Cells
Jiying Liang1, Han Jia1, Lan Li1
1Department of Chemistry, The University of Hong Kong, Pokfulam Road, HK SAR, China.
Organic Letters
|June 2, 2021
Abstract:
We designed β-difluoroalkylamine to capture RNAs and proteins with high tempospatial resolution via proximity labeling mediated by photoinduced singlet oxygen. The appended azide group allows for RNA biotinylation and downstream analysis through both SPAAC and CuAAC. In particular, the β-difluoroalkylazide motif enjoys an enhanced CuAAC reaction rate, thus preserving good RNA integrity.
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