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[Effect of pancuronium bromide on the adherence of polymorphonuclear neutrophilic granulocytes in vitro]
W Krumholz1, S Käbisch, J Biscoping
1Abteilung für Anaesthesiologie und operative Intensivmedizin, Klinikum der Justus-Liebig-Universität Giessen.
Abstract:
Infection is still an important problem following surgery. Polymorphonuclear neutrophil granulocytes (PMN) play a vital role in host defenses against invading bacteria; thus, adverse effects on PMN caused by anesthetic agents are of general interest. In this study, we examined the influence of pancuronium bromide on PMN adherence in vitro. Heparinized venous blood samples were obtained from 18 healthy male adult donors. Each specimen was divided. One part was used to determine the adherence of untreated PMN, while pancuronium bromide was added to the other. Two concentrations (1.3 or 0.5 micrograms relaxant/ml blood) were tested. PMN adherence was studied using nylon fiber columns as described by MacGregor et al. Both concentrations of pancuronium bromide caused an inhibition of adherence, which was significant in the case of the higher dosage (p less than 0.05). There is a direct correlation between the intensity of PMN adherence and the extent of granulocyte delivery to sites of inflammation. Therefore, it seems possible that clinical concentrations of pancuronium bromide may be able to increase the risk of developing bacterial infections. Further studies concerning this problem should be conducted.
Insights
Pancuronium bromide significantly inhibits polymorphonuclear neutrophil (PMN) adherence in vitro. This effect may increase the risk of surgical infections by impairing the immune response to bacteria.
Area of Science:
- Immunology
- Pharmacology
- Surgical Research
Context:
- Surgical site infections remain a significant postoperative complication.
- Polymorphonuclear neutrophils (PMN) are crucial for combating bacterial invasion.
- Anesthetic agents' impact on immune cell function is of clinical importance.
Purpose:
- To investigate the in vitro effect of pancuronium bromide on PMN adherence.
- To determine if pancuronium bromide alters the function of neutrophils.
Summary:
- Heparinized blood from healthy donors was used to assess PMN adherence with and without pancuronium bromide.
- Two concentrations of pancuronium bromide (0.5 and 1.3 µg/ml) were tested.
- Both concentrations inhibited PMN adherence, with the higher dose showing a statistically significant reduction (p < 0.05).
Impact:
- Pancuronium bromide may impair granulocyte delivery to inflammatory sites.
- Clinical concentrations of this neuromuscular blocker could potentially elevate the risk of postoperative bacterial infections.
- Further research is warranted to confirm these findings in a clinical setting.