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Removal of contaminating nucleoside diphosphates from commercial preparations of uridine diphosphoglucose
W H Outlaw1, D R Hite, G B Fiore
1Department of Biological Science, Florida State University, Tallahassee 32306-3050.
Analytical Biochemistry
|May 15, 1988
Abstract:
Nucleoside diphosphate contamination in commercial preparations of UDP-Glc poses potential problems in activity assays for enzymes that use this substrate. For removing these contaminants, we report a simple, inexpensive, and rapid method that obviates the need for uncertain corrections in assay calculations.