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A filtration assay for solubilized prolactin receptors using polyethyleneimine-treated membrane filters
1Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Analytical Biochemistry
|June 1, 1988
Abstract:
Free 125I-labeled ovine prolactin can be separated from detergent-solubilized prolactin-receptor complex by filtration on triacetate membrane filters pretreated with polyethyleneimine. Up to 98% of the total 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate solubilized prolactin-receptor complexes from rat liver bound to polyethyleneimine-treated membranes. This simple and rapid technique can be used to quantitate solubilized prolactin-receptor complexes.