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NUSAP1 Accelerates Osteosarcoma Cell Proliferation and Cell Cycle Progression via Upregulating CDC20 and Cyclin A2
Haoran Wang1, Zixiang Liu1, Peng Wu1
1Department of Orthopedics, Hangzhou Children's Hospital, Hangzhou, People's Republic of China.
Oncotargets and Therapy
|June 3, 2021
Summary
Nucleolar and spindle-associated protein 1 (NUSAP1) promotes osteosarcoma development by accelerating cell proliferation and cell cycle. Targeting NUSAP1 may offer a new therapeutic strategy for osteosarcoma treatment.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Nucleolar and spindle-associated protein 1 (NUSAP1) is a key mitotic regulator implicated in various cancers.
- Its specific role in osteosarcoma pathogenesis requires further elucidation.
Purpose of the Study:
- To investigate the functional role of NUSAP1 in osteosarcoma.
- To explore the underlying molecular mechanisms of NUSAP1 in osteosarcoma progression.
Main Methods:
- Quantitative polymerase chain reaction (RT-qPCR) and Western blot assays assessed NUSAP1, CDC20, and CCNA2 expression.
- Cell proliferation assays (CCK-8, EdU) and flow cytometry analyzed cell cycle progression.
- An osteosarcoma mouse model and immunoprecipitation identified NUSAP1 interactions.
Main Results:
- NUSAP1 was significantly upregulated in osteosarcoma cells.
- NUSAP1 overexpression enhanced cell proliferation and cell cycle, while silencing suppressed these processes.
- NUSAP1 affected tumor growth and proliferation markers (Ki67, PCNA) in vivo.
- Silencing CDC20 or CCNA2 counteracted NUSAP1-induced proliferation and cell cycle acceleration.
Conclusions:
- Upregulated NUSAP1 exacerbates osteosarcoma development by promoting proliferation and cell cycle.
- NUSAP1 interacts with CDC20 and CCNA2 to drive these effects.
- NUSAP1 presents a potential therapeutic target for osteosarcoma treatment.
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