Related Experiment Video

Updated: Nov 3, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.6K

Silencing HIPPI Suppresses Tumor Progression in Non-Small-Cell Lung Cancer by Inhibiting DNA Replication

Guanghui Xie1,2, Yongwen Li1, Yongjun Jiang2

  • 1Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin, People's Republic of China.

Oncotargets and Therapy
|June 3, 2021
PubMed
Abstract

Insights

Huntingtin interacting protein-1 interacting protein (HIPPI) is highly expressed in non-small cell lung cancer (NSCLC). Silencing HIPPI inhibits NSCLC growth, migration, and invasion, suggesting HIPPI as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Non-small cell lung cancer (NSCLC) is the predominant form of lung cancer.
  • Huntingtin interacting protein-1 interacting protein (HIPPI) regulates transcription and apoptosis.
  • The specific role of HIPPI in NSCLC pathogenesis was previously undefined.

Purpose of the Study:

  • To investigate the expression and function of HIPPI in non-small cell lung cancer.
  • To determine the potential of HIPPI as a therapeutic target for NSCLC.

Main Methods:

  • Immunohistochemistry (IHC) and qRT-PCR for HIPPI expression analysis.
  • In vitro assays (CCK-8, colony formation, flow cytometry, wound healing, Transwell invasion) and in vivo mouse xenograft models to assess HIPPI's functional role.
  • Gene microarray and bioinformatics analysis to identify downstream targets of HIPPI.

Main Results:

  • HIPPI expression is significantly elevated in NSCLC tissues compared to normal adjacent tissues.
  • HIPPI silencing via RNA interference suppressed NSCLC cell proliferation, migration, and invasion in vitro and reduced tumor growth in vivo.
  • HIPPI knockdown enhanced sensitivity to cisplatin treatment in NSCLC cells.
  • HIPPI positively regulates MCM2, MCM6, and MCM8, key DNA replication factors, which are also upregulated in NSCLC.

Conclusions:

  • HIPPI plays a critical role in NSCLC tumor biology.
  • HIPPI represents a promising therapeutic target for the treatment of non-small cell lung cancer.
Keywords:
DNA replicationHIPPIMCMnon-small cell lung cancer

More Related Videos

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.2K
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

20.2K

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.7K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.4K
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.8K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.4K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.1K