Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inclusion of a recovery period after pulse treatment in the primary human lymphocyte micronucleus assay covers potential cell cycle delays without loss of sensitivity.

Mutagenesis·2026
Same author

Evidence of erythropoiesis rather than direct genotoxicity causing micronucleus induction by a new protoporphyrinogen-oxidase inhibitor.

Mutation research. Genetic toxicology and environmental mutagenesis·2025
Same author

Smoking Topography, Nicotine Kinetics, and Subjective Smoking Experience of Mentholated and Non-Mentholated Heated Tobacco Products in Occasional Smokers.

Toxics·2025
Same author

Characteristics of a tattooed population and a possible role of tattoos as a risk factor for chronic diseases: Results from the LIFE-Adult-Study.

PloS one·2025
Same author

Applicability of In Silico New Approach Methods for the Risk Assessment of Tattoo Ink Ingredients.

Environmental and molecular mutagenesis·2025
Same author

Disposable e-cigarettes and their nicotine delivery, usage pattern, and subjective effects in occasionally smoking adults.

Scientific reports·2025
Same journal

How genotoxic is the indoor air environment?

Mutagenesis·2026
Same journal

Unveiling the Genotoxic Safety and Antioxidant-Mediated DNA Protective Mechanisms of Triammonium Citrate: An Integrated In Vitro and In Silico Approach.

Mutagenesis·2026
Same journal

Nutritional maternal factors affect the frequency of DNA damage in newborns.

Mutagenesis·2026
Same journal

Potassium bromate as a probable human Carcinogen: multi-organ toxicity and chemopreventive potential of dietary antioxidants-a comprehensive review.

Mutagenesis·2026
Same journal

DNA damage in the leukocytes of nanocomposite-exposed workers.

Mutagenesis·2026
Same journal

Ultra-Processed Foods and Diet Quality in Long COVID: Associations with Symptom Burden, DNA Damage, and Inflammation.

Mutagenesis·2026
See all related articles

Related Experiment Video

Updated: Nov 3, 2025

An In Ovo Model for Testing Insulin-mimetic Compounds
06:09

An In Ovo Model for Testing Insulin-mimetic Compounds

Published on: April 23, 2018

10.8K

The hen's egg test for micronucleus induction (HET-MN): validation data set.

Kerstin Reisinger1, Dagmar Fieblinger2, Andreas Heppenheimer3

  • 1Henkel AG & Co KGaA, Duesseldorf, Germany.

Mutagenesis
|June 3, 2021
PubMed
Summary
This summary is machine-generated.

The hen's egg test for micronucleus induction (HET-MN) offers a sensitive method for genotoxicity testing. This assay addresses limitations of traditional in vitro tests, showing high accuracy in validation studies.

More Related Videos

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
12:56

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

Published on: May 13, 2019

22.8K
Author Spotlight: Cryopreservation of Whole Blood Samples for High Throughput Analysis
06:14

Author Spotlight: Cryopreservation of Whole Blood Samples for High Throughput Analysis

Published on: February 23, 2024

4.5K

Related Experiment Videos

Last Updated: Nov 3, 2025

An In Ovo Model for Testing Insulin-mimetic Compounds
06:09

An In Ovo Model for Testing Insulin-mimetic Compounds

Published on: April 23, 2018

10.8K
An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
12:56

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

Published on: May 13, 2019

22.8K
Author Spotlight: Cryopreservation of Whole Blood Samples for High Throughput Analysis
06:14

Author Spotlight: Cryopreservation of Whole Blood Samples for High Throughput Analysis

Published on: February 23, 2024

4.5K

Area of Science:

  • Toxicology
  • Genetics
  • Biotechnology

Background:

  • Classical in vitro genotoxicity tests show high sensitivity but also a significant rate of misleading positives.
  • Limitations exist in standard 2D cell cultures regarding in vivo relevance, specifically route of exposure and metabolic capacity.
  • The need for improved genotoxicity assays that better reflect in vivo conditions is critical for regulatory science.

Purpose of the Study:

  • To validate the hen's egg test for micronucleus induction (HET-MN) as a reliable genotoxicity assay.
  • To assess the HET-MN's performance in terms of sensitivity, specificity, and accuracy.
  • To provide a follow-up assay for positive findings from classical in vitro genotoxicity tests.

Main Methods:

  • Development of the hen's egg test for micronucleus induction (HET-MN) using fertilized chicken eggs and micronucleus frequency in erythrocytes.
  • A double-blinded validation study involving over 30 chemicals tested across three independent laboratories.
  • Utilized classical readout parameters for genotoxicity assessment.

Main Results:

  • The HET-MN demonstrated high specificity (98%) and sensitivity (84%), resulting in an overall accuracy of 91%.
  • The assay effectively mimics systemic compound availability after oral exposure, including Absorption, Distribution, Metabolism, and Excretion (ADME) aspects.
  • The test is not classified as an animal experiment, offering an ethical advantage.

Conclusions:

  • The HET-MN is a validated and accurate genotoxicity assay that overcomes key limitations of traditional in vitro methods.
  • This assay serves as a valuable addition to the genotoxicity testing toolbox, particularly for follow-up investigations.
  • The HET-MN provides a relevant model for assessing genotoxic potential with improved in vivo correlation.