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Updated: Nov 3, 2025

Ultrasound-guided Intracardiac Injection of Human Mesenchymal Stem Cells to Increase Homing to the Intestine for Use in Murine Models of Experimental Inflammatory Bowel Diseases
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Ultrasound-guided Intracardiac Injection of Human Mesenchymal Stem Cells to Increase Homing to the Intestine for Use in Murine Models of Experimental Inflammatory Bowel Diseases

Published on: September 1, 2017

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MRISCs protect colonic stem cells from inflammatory damage

Guoli Zhu1, Rongwen Xi2,3

  • 1National Institute of Biological Sciences, No. 7 Science Park Road, Zhongguancun Life Science Park, Beijing, 102206, China.

Cell Regeneration (London, England)
|June 3, 2021
PubMed

Insights

Researchers discovered a new cell type in the colon, MAP3K2-Regulated Intestinal Stromal Cells (MRISCs), which protect intestinal stem cells during inflammation by expressing R-spondin1.

Area of Science:

  • Gastroenterology
  • Cell Biology
  • Immunology

Background:

  • Subepithelial mesenchymal niche cells are crucial for intestinal stem cell maintenance and disease modulation.
  • The specific roles of these cells in intestinal homeostasis and disease pathogenesis are under active investigation.

Purpose of the Study:

  • To identify and characterize novel stromal cell populations in the mammalian intestine.
  • To elucidate the function of these newly discovered cells in protecting intestinal stem cells during inflammation.

Main Methods:

  • Utilized advanced single-cell RNA sequencing and spatial transcriptomics in mouse models.
  • Investigated the expression patterns and functional roles of identified stromal cell populations.

Main Results:

  • Discovered a new population of stromal cells in the mouse colon, termed MAP3K2-Regulated Intestinal Stromal Cells (MRISCs).
  • MRISCs are located at the base of colonic crypts and express the Wnt agonist R-spondin1 (Rspo1).
  • MRISCs demonstrate a protective role for colonic stem cells during inflammatory conditions.

Conclusions:

  • MRISCs represent a critical component of the intestinal stem cell niche.
  • These cells play a significant role in mitigating intestinal inflammation and preserving stem cell function.

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