Quantitatively Controlled Intercellular Mitochondrial Transfer by Cell Fusion-Based Method Using a Microfluidic
Ken-Ichi Wada1,2, Kazuo Hosokawa3, Yoshihiro Ito4
1Bioengineering Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama, Japan. ki-wada@riken.jp.
Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2021
Abstract:
Quantitative control of mitochondrial transfer is a promising approach for genetic manipulation of mitochondrial DNA (mtDNA) because it enables precise modulation of heteroplasmy. Furthermore, single mitochondrion transfer from a mtDNA mutation-accumulated cell to a mtDNA-less (ρ0) cell potentially achieves homoplasmy of mutated mtDNA. Here we describe the method for quantitative control of mitochondrial transfer including achieving single mitochondrion transfer between live single cells using a microfluidic device.
Keywords:
Cell fusionHeteroplasmyHomoplasmyMicrofluidicsMicrotunnelMitochondrial DNAMitochondrial transfer
