Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Is it possible to generate artificial mitochondrial genome?]

Nihon yakurigaku zasshi. Folia pharmacologica Japonica·2026
Same author

Copying a soft lithography master mold using an inexpensive, hobby-use UV-curable resin.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Nucleic acid detection based on single-cluster analysis of cross-linking aggregates of DNA-modified gold nanoparticles using a dark-field microscope.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Development of surface-functionalized power-free microchip for breast cancer cell-derived extracellular vesicle detection.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2025
Same author

Protocol for generation of transmitochondrial cybrids under pyruvate/uridine-supplemented conditions using a microfluidic device.

STAR protocols·2025
Same author

Density and structure of DNA immobilised on gold nanoparticles affect sensitivity in nucleic acid detection.

Scientific reports·2025

Related Experiment Video

Updated: Nov 3, 2025

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy
10:45

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

Published on: July 20, 2012

16.8K

Quantitatively Controlled Intercellular Mitochondrial Transfer by Cell Fusion-Based Method Using a Microfluidic

Ken-Ichi Wada1,2, Kazuo Hosokawa3, Yoshihiro Ito4

  • 1Bioengineering Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama, Japan. ki-wada@riken.jp.

Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2021
PubMed
Summary
This summary is machine-generated.

Precise control over mitochondrial transfer, including single mitochondrion delivery, allows for accurate manipulation of mitochondrial DNA (mtDNA) heteroplasmy in cells. This technique offers a new way to achieve homoplasmy for genetic studies.

Keywords:
Cell fusionHeteroplasmyHomoplasmyMicrofluidicsMicrotunnelMitochondrial DNAMitochondrial transfer

More Related Videos

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines
07:49

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Published on: March 17, 2023

2.8K
Author Spotlight: Bidirectional Mitochondrial Transfer between MSCs and Retinal Pigment Epithelium Cells — Pathways and In Vivo Challenges
06:50

Author Spotlight: Bidirectional Mitochondrial Transfer between MSCs and Retinal Pigment Epithelium Cells — Pathways and In Vivo Challenges

Published on: October 4, 2024

1.2K

Related Experiment Videos

Last Updated: Nov 3, 2025

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy
10:45

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

Published on: July 20, 2012

16.8K
Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines
07:49

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Published on: March 17, 2023

2.8K
Author Spotlight: Bidirectional Mitochondrial Transfer between MSCs and Retinal Pigment Epithelium Cells — Pathways and In Vivo Challenges
06:50

Author Spotlight: Bidirectional Mitochondrial Transfer between MSCs and Retinal Pigment Epithelium Cells — Pathways and In Vivo Challenges

Published on: October 4, 2024

1.2K

Area of Science:

  • Cell Biology
  • Genetics
  • Bioengineering

Background:

  • Mitochondrial DNA (mtDNA) mutations are linked to various diseases.
  • Precise control over mtDNA levels (heteroplasmy) is crucial for understanding and potentially treating these conditions.
  • Current methods for manipulating mtDNA are often imprecise.

Purpose of the Study:

  • To develop a method for quantitative control of mitochondrial transfer between cells.
  • To enable the transfer of single mitochondria for precise genetic manipulation.
  • To achieve homoplasmy of mutated mtDNA in recipient cells.

Main Methods:

  • Utilized a microfluidic device for controlled manipulation of live single cells.
  • Developed protocols for quantitative mitochondrial transfer.
  • Demonstrated the capability of transferring single mitochondria.

Main Results:

  • Successfully achieved quantitative control over mitochondrial transfer.
  • Demonstrated the ability to transfer individual mitochondria between live cells.
  • Showcased the potential for achieving mtDNA homoplasmy in recipient cells.

Conclusions:

  • Quantitative mitochondrial transfer, especially single mitochondrion transfer, is a viable strategy for precise mtDNA manipulation.
  • This method provides a powerful tool for studying mtDNA genetics and developing novel therapeutic approaches.
  • Microfluidic technology enables unprecedented control over cellular components for genetic engineering.