Related Experiment Video
Updated: Nov 3, 2025
03:57
Assessment of Mitochondrial Oxygen Consumption Using a Plate Reader-based Fluorescent Assay
Published on: April 12, 2024
810
In Vivo Assessment of Mitochondrial Oxygen Consumption
1Department of Anesthesiology, Laboratory of Experimental Anesthesiology, Erasmus MC - University Medical Center Rotterdam, Rotterdam, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2021
Abstract:
The Protoporphyrin IX-Triplet State Lifetime Technique (PpIX-TSLT) has been proposed by us as a potential clinical noninvasive tool for monitoring mitochondrial function. We have been working on the development of mitochondrial respirometry for monitoring mitochondrial oxygen tension (mitoPO2) and mitochondrial oxygen consumption (mitoVO2) in skin. In this work, we describe the principles of the method in small experimental animals.
