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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Published on: March 1, 2019
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Transcriptome wide analysis of long non-coding RNA-associated ceRNA regulatory circuits in psoriasis.
Jingxia Lin1, Xuefei Li1, Fangfei Zhang1
1Dermatology Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Journal of Cellular and Molecular Medicine
|June 3, 2021
Summary
Long non-coding RNAs (lncRNAs) are implicated in psoriasis pathogenesis. This study reveals their role in regulating the JAK/STAT pathway, offering potential therapeutic targets for this chronic inflammatory disease.
Area of Science:
- Immunology
- Genetics
- Molecular Biology
Background:
- Long non-coding RNAs (lncRNAs) are crucial regulators in immune and inflammatory diseases.
- Psoriasis is a chronic inflammatory skin condition with complex underlying mechanisms.
Purpose of the Study:
- To investigate the role of lncRNAs in psoriasis pathogenesis.
- To elucidate the molecular mechanisms by which lncRNAs regulate inflammation in psoriasis.
- To identify potential therapeutic targets for psoriasis.
Main Methods:
- Integrative analysis of RNA-sequencing data from psoriasis patients.
- lncRNA-protein-coding gene co-expression network construction.
- Experimental validation of lncRNA function in IFN-γ stimulated HaCaT cells.
- MicroRNA target screening and mechanistic studies of lncRNA-mediated regulation.
Main Results:
- lncRNAs are involved in psoriasis pathogenesis and extensively interact with IFN-γ signaling pathway genes.
- Three specific lncRNAs were validated to associate with IFN-γ signaling pathway activation and regulate inflammatory cytokine genes.
- lncRNAs were shown to modulate JAK/STAT pathway activity via a competing endogenous RNA (ceRNA) mechanism, exemplified by the PRKCQ-AS1/STAT1/miR-545-5p axis.
Conclusions:
- Dysregulation of the lncRNA-JAK/STAT pathway axis contributes to elevated inflammation in psoriasis.
- Targeting the lncRNA-JAK/STAT pathway axis presents a promising therapeutic strategy for psoriasis treatment.
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