Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing
Cedric Woudstra1, Tommi Mäklin2, Yagmur Derman1
1Department of Food Hygiene and Environmental Health, University of Helsinki, Helsinki, Finland.
Abstract:
Clostridium botulinum group III is the anaerobic Gram-positive bacterium producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol for obtaining high-molecular-weight DNA from C. botulinum group III is described.
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