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Endophytes isolated from Panax notoginseng converted ginsenosides
Guangfei Wei1, Zhongjian Chen2, Bo Wang3
1Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16 Nanxiaojie, Dongzhimennei Ave, Beijing, 100700, China.
Microbial Biotechnology
|June 3, 2021
Summary
Medicinal plant endophytes, like Enterobacter chengduensis and Penicillium chermesinum, can convert saponins. This study maps endophyte distribution in P. notoginseng and confirms their role in metabolite conversion.
Area of Science:
- Microbiology
- Plant Science
- Metabolomics
Background:
- Endophytes influence host plant metabolites and efficacy.
- The distribution and function of endophytes in P. notoginseng, particularly in saponin conversion, remain unclear.
Purpose of the Study:
- To determine endophyte and saponin distribution within P. notoginseng compartments.
- To confirm endophyte-mediated saponin conversion within the host plant.
Main Methods:
- Compartmental analysis of saponins and endophytes in P. notoginseng.
- Microbial diversity assessment (alpha diversity, composition, networks).
- Spearman correlation analysis and in vitro verification of endophyte-saponin conversion.
Main Results:
- Saponin metabolites and endophyte communities exhibited compartment-specific distribution.
- Bacterial diversity peaked in roots, while fungal diversity was highest in leaves.
- Specific endophytes, including Enterobacter chengduensis, Trichoderma koningii, and Penicillium chermesinum, demonstrated conversion of key saponins (Rg1, Rb1) into other compounds (F1, Rd, Rg3).
Conclusions:
- Endophyte distribution and composition are compartment-specific in P. notoginseng.
- Certain endophytes possess the capability to convert major saponins, potentially modulating host plant efficacy.
