Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.8K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Active Templating by Mechanochemistry.

Organic letters·2026
Same author

Sequence-Dependent Folding of Recognition-Encoded Melamine Oligomers.

Journal of the American Chemical Society·2026
Same author

Novel macrocycles: from synthesis to supramolecular function.

Beilstein journal of organic chemistry·2026
Same author

Balancing acceleration and turnover in [1 + 1] tetra-imine bis-calix[4]pyrrole reactor for Huisgen cycloadditions.

Nature communications·2026
Same author

Negative cooperativity in the formation of two H-bonds with an oxygen H-bond acceptor.

Chemical science·2026
Same author

Dissecting halide-receptor interactions in "four wall" aryl-extended calix[4]pyrrole complexes: the role of the aromatic walls.

Physical chemistry chemical physics : PCCP·2026

Related Experiment Video

Updated: Nov 3, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.6K

High-Fidelity Sequence-Selective Duplex Formation by Recognition-Encoded Melamine Oligomers.

Pavle Troselj1, Peter Bolgar1, Pablo Ballester2,3

  • 1Yusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.

Journal of the American Chemical Society
|June 3, 2021
PubMed
Summary

Melamine oligomers with specific side-chains reliably form stable H-bonded duplexes due to their rigid backbone. This self-assembly property is sequence-dependent, making them promising for molecular construction.

More Related Videos

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

9.7K
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

670

Related Experiment Videos

Last Updated: Nov 3, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.6K
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

9.7K
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

670

Area of Science:

  • Supramolecular chemistry
  • Polymer chemistry
  • Organic chemistry

Background:

  • Melamine-based oligomers with phenol and phosphine oxide side-chains are designed for self-assembly.
  • Hydrogen-bonded duplex formation is a key feature of these oligomers.

Purpose of the Study:

  • To investigate the self-assembly properties of melamine oligomers.
  • To characterize the stability and fidelity of duplex formation.

Main Methods:

  • Nuclear Magnetic Resonance (NMR) spectroscopy to analyze molecular structure and folding.
  • Isothermal Titration Calorimetry (ITC) to quantify binding affinities and thermodynamic parameters.

Main Results:

  • Melamine oligomers form stable H-bonded duplexes, with stability increasing significantly with chain length.
  • Oligomer backbone rigidity prevents intramolecular folding, ensuring reliable intermolecular duplex formation.
  • Sequence-complementary duplexes are approximately 10 times more stable than those with single mismatches.

Conclusions:

  • Melamine oligomers exhibit high-fidelity duplex formation driven by sequence complementarity.
  • The synthetic accessibility and predictable self-assembly make these systems attractive for advanced molecular materials.