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Boosting transfection efficiency: A systematic study using layer-by-layer based gene delivery platform.

Yana V Tarakanchikova1, Dmitrii S Linnik2, Tatiana Mashel3

  • 1Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya, 29, 195251 St. Petersburg, Russian Federation; Nanobiotechnology Laboratory, St. Petersburg Academic University, 194021 St. Petersburg, Russian Federation.

Materials Science & Engineering. C, Materials for Biological Applications
|June 4, 2021
PubMed
Summary
This summary is machine-generated.

Optimized nanoparticle multilayer structures significantly enhance gene delivery efficiency for gene therapy. This study details key parameters for high transfection rates and a good safety profile, offering a promising non-viral nucleic acid delivery platform.

Keywords:
Calcium carbonateCell incubation protocolCore-shell particlesDNase inhibitorGene deliveryOrganic additives

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Area of Science:

  • Biotechnology
  • Gene Therapy
  • Nanomedicine

Background:

  • Nanoparticle-based delivery is attractive for gene therapy due to low toxicity and efficient production.
  • Multilayer structures show potential for plasmid DNA (pDNA) delivery, but optimization is needed.
  • Systematic studies on optimizing pDNA delivery using multilayer structures are lacking.

Purpose of the Study:

  • To systematically analyze parameters influencing pDNA delivery efficiency using multilayered inorganic-polymer nanoparticles.
  • To investigate the impact of gene loading strategies, organic additives, and incubation conditions on transfection efficiency.
  • To develop optimized protocols for enhanced gene delivery via non-viral platforms.

Main Methods:

  • Fabrication of multilayered nanoparticles with inorganic cores and polymer layers.
  • Systematic variation of pDNA loading strategies and incorporation of organic additives.
  • Optimization of cell/particle incubation conditions for pDNA delivery.

Main Results:

  • An optimized combination of parameters significantly increased transfection efficiency (>70%) compared to non-optimized methods (<7%).
  • The developed multilayered structures demonstrated a favorable safety profile.
  • Proof-of-principle for optimized multilayer structures as an efficient non-viral gene delivery system.

Conclusions:

  • Optimized parameters are crucial for maximizing the efficiency of multilayered nanoparticle-mediated gene delivery.
  • These multilayered structures represent a promising non-viral platform for nucleic acid delivery in gene therapy.
  • The developed protocols offer a scalable and effective approach for enhanced gene transfection.