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Updated: Nov 3, 2025

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
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Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Published on: June 9, 2022

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Sense codon reassignment enables viral resistance and encoded polymer synthesis.

Wesley E Robertson1, Louise F H Funke1, Daniel de la Torre1

  • 1Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.

Science (New York, N.Y.)
|June 4, 2021
PubMed
Summary

Scientists engineered Escherichia coli to remove transfer RNAs (tRNAs), creating a genetic firewall against viruses. This breakthrough enables the efficient synthesis of proteins with novel noncanonical amino acids.

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