Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Protein Maturation01:26

Bacterial Protein Maturation

181
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
181
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

12.9K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
12.9K
The Cell Cycle Control System01:28

The Cell Cycle Control System

4.4K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
4.4K
The Cell Cycle Control System02:11

The Cell Cycle Control System

13.0K
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
13.0K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.4K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.4K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

3.0K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular Solution to the Paradox of Ancient Brain Preservation.

Journal of proteome research·2026
Same author

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026
Same author

NMDAR-antibody encephalitis: Seizure semiology and EEG findings.

Epileptic disorders : international epilepsy journal with videotape·2026
Same author

Psychiatric and behavioural sequelae following encephalitis: a systematic review and meta-analysis.

Brain communications·2026
Same author

USP24 is a cross-reactive DUB targeting MOV10 to regulate IFN-I production.

Nature communications·2026
Same author

Multidimensional proteomics and explainable AI feature selection identify cross-platform lung cancer molecular signature in blood plasma.

Communications medicine·2026

Related Experiment Video

Updated: Nov 3, 2025

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
06:55

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Published on: June 7, 2020

3.1K

The chaperonin CCT8 controls proteostasis essential for T cell maturation, selection, and function.

Bergithe E Oftedal1,2, Stefano Maio1, Adam E Handel1

  • 1Developmental Immunology, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, OX3 9DS, UK.

Communications Biology
|June 4, 2021
PubMed
Summary

The eukaryotic chaperonin complex, CCT, is essential for T cell development and function. Its absence impairs T cell proteostasis, leading to compromised immune responses and an inability to mount effective immunity.

More Related Videos

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms
09:41

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms

Published on: July 26, 2024

1.1K
Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
12:38

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

Published on: December 18, 2013

6.3K

Related Experiment Videos

Last Updated: Nov 3, 2025

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
06:55

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Published on: June 7, 2020

3.1K
Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms
09:41

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms

Published on: July 26, 2024

1.1K
Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
12:38

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

Published on: December 18, 2013

6.3K

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • T cells require proper protein folding and turnover for development and function.
  • The eukaryotic type II chaperonin complex, CCT, plays a crucial role in cellular proteostasis.

Purpose of the Study:

  • To investigate the role of CCT in T cell development, function, and immune response.
  • To understand the consequences of CCT absence on T cell proteome dynamics and immune signaling.

Main Methods:

  • Analysis of T cell activation and differentiation in the absence of CCT.
  • Assessment of proteome changes, nuclear actin filament formation, and stress response.
  • Evaluation of Th2 polarization, IFN-γ expression, and immune protection against helminths.

Main Results:

  • Absence of CCT compromises T cell activation-induced proteome changes, including nuclear actin formation and stress response.
  • Thymocyte maturation, selection, and T cell homeostasis are severely impaired without CCT.
  • CCT-deficient T cells show aberrant Th2 polarization with persistent IFN-γ expression, failing to coordinate innate and adaptive immunity for effective helminth protection.

Conclusions:

  • Normal T cell biology is critically dependent on CCT-controlled proteostasis.
  • The absence of CCT disrupts T cell function and is incompatible with protective immunity.