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Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line

Dolly Patricia Pardo-Mora1, Oscar Julián Murillo2, Mauricio Rey-Buitrago1,2

  • 1Department of Animal Health, Facultad de Medicina Veterinaria, Universidad Antonio Nariño, Bogotá, Colombia.

Veterinary World
|June 4, 2021
PubMed
Abstract

Insights

Colombian propolis demonstrated cytotoxic and apoptotic effects on canine osteosarcoma cells. Gene expression analysis revealed propolis induces apoptosis, particularly via the extrinsic pathway involving CASPASE 8.

Area of Science:

  • Natural Products Research
  • Oncology
  • Molecular Biology

Background:

  • Osteosarcoma (OSA) is the most common bone tumor in canines.
  • Investigating natural compounds for cancer therapy is crucial.

Purpose of the Study:

  • To evaluate the cytotoxic and apoptotic effects of Colombian propolis on canine OSA cells (OSCA-8).
  • To analyze the expression of key apoptosis-related genes (BCL-2, BAX, CASPASE 9, CASPASE 8, TNFR1).

Main Methods:

  • Treatment of OSCA-8 cells with five Colombian propolis samples.
  • Assessment of cell viability and lactate dehydrogenase (LDH) release.
  • Flow cytometry for apoptosis determination and real-time PCR for gene expression analysis.

Main Results:

  • Propolis samples reduced cell viability and increased LDH release.
  • All samples significantly induced apoptosis.
  • Upregulation of BCL-2, CASPASE 8, BAX, CASPASE 9, and TNFR1 gene expression was observed with specific samples.

Conclusions:

  • Colombian propolis exhibits significant cytotoxic and apoptotic effects on canine osteosarcoma cells.
  • CASPASE 8 upregulation suggests apoptosis induction through the extrinsic pathway.
Keywords:
apoptosiscytotoxicityosteosarcomapropolis

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