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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

849
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
849

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Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
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Raman-Enhanced Spectroscopy (RESpect) Probe for Childhood Non-Hodgkin Lymphoma.

Melissa Agsalda-Garcia1, Tiffany Shieh1, Ryan Souza1

  • 1Department of Tropical Medicine, Medical Microbiology, and Pharmacology, University of Hawaii, Hawaii, United States.

Scimedicine Journal
|June 4, 2021
PubMed
Summary

A portable Raman-enhanced spectroscopy (RESpect) probe shows promise for identifying pediatric non-Hodgkin lymphoma. This technology could lead to new diagnostic approaches and potential therapeutic targets for cancer screening.

Keywords:
LymphomaNon-Hodgkin LymphomaRaman Spectroscopy

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Area of Science:

  • Biomedical Engineering
  • Oncology
  • Spectroscopy

Background:

  • Raman spectroscopy (RS) is a valuable technique for molecular characterization of tissues.
  • Portable fiber-optic devices can enhance RS accessibility for clinical applications.
  • Distinguishing between malignant and non-malignant tissues is crucial for diagnosis and treatment.

Purpose of the Study:

  • To evaluate the feasibility of a Raman-enhanced spectroscopy (RESpect) probe for pediatric non-Hodgkin lymphoma diagnosis.
  • To compare the performance of the RESpect probe with standard Raman spectroscopy microscopy.
  • To explore the potential of RESpect as a point-of-care screening instrument.

Main Methods:

  • Analysis of cryopreserved pediatric non-Hodgkin lymphoma and non-malignant tissues using standard RS and the RESpect probe.
  • Utilizing Principal Component Analysis (PCA) to differentiate tissue types based on spectral data.
  • Comparing Raman spectroscopic fingerprints and primary peaks obtained from both methods.

Main Results:

  • Principal Component Analysis revealed distinct spectral similarities among non-Hodgkin lymphoma subtypes.
  • Follicular hyperplasia showed different spectral patterns compared to non-Hodgkin lymphoma subtypes.
  • The RESpect probe demonstrated comparable primary peaks to standard Raman spectroscopy, indicating reliable performance.

Conclusions:

  • Raman spectroscopy, particularly with the RESpect probe, offers novel avenues for diagnosing pediatric non-Hodgkin lymphoma.
  • The RESpect probe's portability suggests potential as a point-of-care screening tool, potentially altering cancer screening paradigms.
  • Findings may inform new insights into molecular pathogenesis and identify potential therapeutic targets.