Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

849
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
849
Properties of Enantiomers and Optical Activity02:24

Properties of Enantiomers and Optical Activity

19.5K
It is essential to understand the difference between chiral and achiral interactions and the implications thereof in optical activity and their applications. Just as our feet, which are chiral, interact uniquely with chiral objects, such as a pair of shoes, but identically with achiral socks, enantiomers of a molecule exhibit different properties only when they interact with other chiral media. An example of a significant implication from this facet is the phenomenon known as optical activity,...
19.5K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

613
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
613
Chirality in Nature02:30

Chirality in Nature

15.1K
Chirality is the most intriguing yet essential facet of nature, governing life’s biochemical processes and precision. It can be observed from a snail shell pattern in a macroscopic world to an amino acid, the minutest building block of life. Most of the snails around the world have right-coiled shells because of the intrinsic chirality in their genes. All the amino acids present in the human body exist in an enantiomerically pure state, except for glycine - the sole achiral amino acid.
15.1K
Prochirality02:05

Prochirality

4.4K
The concept of prochirality leads to the nomenclature of the individual faces of a molecule and plays a crucial role in the enantioselective reaction. It is a concept where two or more achiral molecules react to produce chiral products. A typical process is the reaction of an achiral ketone to generate a chiral alcohol. Here, the achiral reactant reacts with an achiral reducing agent, sodium borohydride, to generate an equimolar mixture of the chiral enantiomers of the product. For example, an...
4.4K
Chirality at Nitrogen, Phosphorus, and Sulfur02:30

Chirality at Nitrogen, Phosphorus, and Sulfur

6.4K
Chirality is most prevalent in carbon-based tetrahedral compounds, but this important facet of molecular symmetry extends to sp3-hybridized nitrogen, phosphorus and sulfur centers, including trivalent molecules with lone pairs. Here, the lone pair behaves as a functional group in addition to the other three substituents to form an analogous tetrahedral center that can be chiral.
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultraviolet Raman Optical Activity as a Window Into Peptide Backbone Structure.

Chembiochem : a European journal of chemical biology·2026
Same author

Structural Sensitivity without Chirality: Observation of Magnetic Raman Optical Activity outside Resonance.

Journal of the American Chemical Society·2026
Same author

Studying Collagen Architecture in Solution by Raman Optical Activity Spectroscopy.

Analytical chemistry·2026
Same author

Three Conformations of Polyglutamic Acid Monitored by Vibrational Optical Activity.

Analytical chemistry·2025
Same author

A hydrogen-bond-stabilized chiral tetrakis Eu(III) complex with strong circularly polarized luminescence.

Dalton transactions (Cambridge, England : 2003)·2025
Same author

Black-Box Simulations of Anharmonic Vibrational Chiroptical Spectra: Problems with Property Third Derivatives and the Solvent.

Journal of chemical theory and computation·2025

Related Experiment Video

Updated: Nov 3, 2025

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.8K

Chiral detection by induced surface-enhanced Raman optical activity.

Moumita Das1, Debraj Gangopadhyay2, Jaroslav Šebestík2

  • 1Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Flemingovo náměstí 2, Prague 16610, Czech Republic. bour@uochb.cas.cz and Department of Analytical Chemistry, University of Chemistry and Technology, Technická 5, Prague 16628, Czech Republic.

Chemical Communications (Cambridge, England)
|June 4, 2021
PubMed
Summary

This study introduces a new method combining optical activity and surface-enhanced Raman scattering (SERS) for detecting chiral acids. The technique achieves sensitive detection of chiral molecules at concentrations as low as 10-5 M.

More Related Videos

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
08:13

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Published on: February 19, 2016

9.5K
Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs
03:33

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Published on: November 17, 2023

2.7K

Related Experiment Videos

Last Updated: Nov 3, 2025

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.8K
A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
08:13

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Published on: February 19, 2016

9.5K
Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs
03:33

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Published on: November 17, 2023

2.7K

Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Chiroptical Methods

Background:

  • Combining optical activity with surface-enhanced Raman scattering (SERS) has been a long-standing goal in physical chemistry.
  • Developing sensitive detection methods for chiral molecules is crucial in various scientific fields.

Purpose of the Study:

  • To establish a measurement protocol for detecting chiral acids using SERS.
  • To investigate the mechanism behind the chiral signal generation in the SERS system.

Main Methods:

  • Utilizing silver colloids as substrates for surface enhancement.
  • Employing aromatic linkers that self-assemble into chiral aggregates.
  • Detecting chiral acids at concentrations down to 10-5 M.

Main Results:

  • Demonstrated successful detection of chiral acids using the developed SERS protocol.
  • Explained the mechanism involving linker binding and self-assembly into chiral aggregates on the silver surface.
  • Observed that chirality is dictated by the minor acidic component, following the 'sergeants-and-soldiers' principle.

Conclusions:

  • The developed SERS-based protocol offers a sensitive method for detecting chiral acids, potentially surpassing the sensitivity of techniques like electronic circular dichroism.
  • This approach shows promise for detecting biologically relevant chiral molecules.
  • Future work should focus on refining linker chemistry and experimental conditions to enhance signal specificity for discriminating among different optically active molecules.