Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adaptable centriole biogenesis via the intrinsically disordered protein ALMS1.

Nature communications·2026
Same author

Molecular architecture of the ciliary base in mammalian multiciliated cells.

bioRxiv : the preprint server for biology·2026
Same author

PRECLINICAL ACTIVITY OF THE B7-H3- TARGETING ANTIBODY-DRUG CONJUGATE (ADC) VOBRAMITAMAB DUOCARMAZINE (VOBRA DUO) IN PEDIATRIC SOLID TUMORS.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Targeting Pediatric Glioblastomas by Combining OLIG2 Inhibitor CT-179 with Fractionated Radiation in a Panel of Patient-Derived Orthotopic Xenograft Mouse Models.

International journal of molecular sciences·2026
Same author

The luminal ring protein C2CD3 acts as a radial in-to-out organizer of the distal centriole and appendages.

PLoS biology·2025
Same author

ADAR1 editing is necessary for only a small subset of cytosolic dsRNAs to evade MDA5-mediated autoimmunity.

Nature genetics·2025

Related Experiment Video

Updated: Nov 3, 2025

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
11:05

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Published on: January 7, 2019

9.7K

Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells.

Jonathan M Geisinger1,2, Tim Stearns3,4

  • 1Department of Biology, Stanford University, Stanford, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 4, 2021
PubMed
Summary

This study presents a flow cytometry assay to analyze cell cycle progression after CRISPR/Cas9 genome editing. This method helps overcome challenges in generating large edited cell populations by assessing cell cycle arrest and transfection efficiency.

Keywords:
CRISPR/Cas9Cell cycleClick chemistryFlow cytometryGene editingGenome engineeringImmunofluorescence

More Related Videos

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
08:52

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

19.6K
Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
11:23

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

21.3K

Related Experiment Videos

Last Updated: Nov 3, 2025

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
11:05

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Published on: January 7, 2019

9.7K
Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
08:52

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

19.6K
Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
11:23

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

21.3K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genomics

Background:

  • The CRISPR/Cas9 system is a revolutionary genome editing tool.
  • Efficiently generating large populations of edited cells remains a challenge for certain cell types.
  • CRISPR/Cas9-induced cell cycle arrest is a potential limiting factor for editing efficiency.

Purpose of the Study:

  • To develop and validate a flow cytometry-based assay for assessing cell cycle progression following CRISPR/Cas9 treatment.
  • To evaluate the impact of CRISPR/Cas9 on cell cycle dynamics in various cell lines.
  • To provide a method for optimizing interventions to increase the yield of edited cells.

Main Methods:

  • Development of a flow cytometry assay.
  • Quantification of cell cycle profiles (e.g., G1, S, G2/M phases) after CRISPR/Cas9 treatment.
  • Assessment of Cas9 vector transfection and expression efficiencies.

Main Results:

  • The assay successfully determines cell cycle progression in the presence of CRISPR/Cas9.
  • It allows for the evaluation of transfection and expression efficiencies of Cas9 vectors.
  • The method facilitates the assessment of interventions aimed at improving the number of edited cells.

Conclusions:

  • This flow cytometry assay is a valuable tool for understanding CRISPR/Cas9-mediated cell cycle effects.
  • It aids researchers in troubleshooting and optimizing genome editing protocols.
  • The assay supports the efficient generation of larger populations of CRISPR/Cas9-edited cells.