Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

4.2K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
4.2K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

8.0K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
8.0K
Microtubules in Signaling01:22

Microtubules in Signaling

1.9K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.9K
Microtubules in Cell Motility01:24

Microtubules in Cell Motility

4.0K
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
4.0K
Mechanism of Ciliary Motion01:05

Mechanism of Ciliary Motion

4.2K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
4.2K
Centrosome Duplication02:25

Centrosome Duplication

4.3K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acsl4-mediated Lipid Homeostasis Orchestrates Synaptic and Cognitive Plasticity.

Research square·2026
Same author

Categorical characteristics of parenting concerns and their influencing factors in young and middle-aged breast cancer patients: a latent profile analysis.

Scientific reports·2026
Same author

Response patterns and influencing factors of body image and emotion in postoperative breast cancer patients: A latent profile analysis.

European journal of oncology nursing : the official journal of European Oncology Nursing Society·2026
Same author

Lac-Phe induces hypophagia by inhibiting AgRP neurons in mice.

Nature metabolism·2025
Same author

Control of epithelial tissue organization by mRNA localization.

Nature communications·2025
Same author

Translation, Reliability and Validity of the Chinese Version of the Confidence in Dementia Scale for Clinical Nursing: A Cross-Sectional Study.

International journal of older people nursing·2024

Related Experiment Video

Updated: Nov 3, 2025

Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model
09:53

Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model

Published on: March 28, 2025

721

Analyzing Centrioles and Cilia by Expansion Microscopy.

Dong Kong1, Jadranka Loncarek2

  • 1Laboratory of Protein Dynamics and Signaling, NIH/NCI/CCR, Frederick, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 4, 2021
PubMed
Summary

Expansion microscopy enables high-resolution imaging of cellular structures like centrioles. This method offers an accessible alternative to electron microscopy for analyzing centriole and cilia features in various cell types.

Keywords:
CentrioleCentriole lengthCentrosomeCiliaExpansion microscopy

More Related Videos

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

9.8K
Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

16.2K

Related Experiment Videos

Last Updated: Nov 3, 2025

Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model
09:53

Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model

Published on: March 28, 2025

721
Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

9.8K
Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

16.2K

Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Biophysics

Background:

  • Centrioles are vital microtubule-based organelles essential for centrosome and cilia formation in vertebrates.
  • Traditional electron microscopy is resource-intensive for studying centriole ultrastructure.
  • Expansion microscopy (ExM) offers a cost-effective and accessible alternative for high-resolution imaging.

Purpose of the Study:

  • To present an optimized expansion microscopy protocol for analyzing centrioles and cilia.
  • To demonstrate the application of ExM in diverse mammalian cell models, including adherent, nonadherent, and multiciliated cells.

Main Methods:

  • Isotropic physical expansion of biological samples.
  • High-resolution imaging of expanded cellular structures using conventional microscopes.
  • Application of ExM to analyze centriole and cilia length and ultrastructural features.

Main Results:

  • Successfully applied ExM to analyze centrioles and cilia in large cell populations.
  • Demonstrated the utility of ExM across various mammalian cell types.
  • Provided a detailed approach for structural analysis of centrioles and cilia.

Conclusions:

  • Expansion microscopy is a powerful and accessible tool for studying centriole and cilia biology.
  • This method enhances optical resolution, overcoming limitations of conventional microscopy for subresolutional structures.
  • The described approach facilitates detailed ultrastructural analysis of centrioles and cilia in diverse cellular contexts.