Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

780
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
780
CRISPR01:59

CRISPR

54.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
54.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.0K
RNA Editing02:23

RNA Editing

9.3K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

eIF4E2 deficiency translationally unleashes WRN to sustain DNA repair-mediated chemoresistance.

Cell reports·2026
Same author

Profiling of terminating ribosomes reveals translational control at stop codons.

eLife·2026
Same author

Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

The Journal of cell biology·2026
Same author

p53 overrides <i>METTL5</i> loss-induced tumor suppression via mitochondrial respiration.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Type II tRNA cleavage by SLFN14 endoribonuclease variants linked to inherited thrombocytopenia drives global translational repression.

PLoS biology·2026
Same author

The m<sup>6</sup>A reader protein YTHDF2 facilitates HTLV-1 infectious and mitotic propagation by stabilizing Tax RNA.

Journal of virology·2026

Related Experiment Video

Updated: Nov 3, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.9K

Targeted RNA m6A Editing Using Engineered CRISPR-Cas9 Conjugates.

Xiao-Min Liu1, Shu-Bing Qian2

  • 1School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.

Methods in Molecular Biology (Clifton, N.J.)
|June 4, 2021
PubMed
Summary

Researchers developed a CRISPR-Cas9 tool to precisely add or remove N6-methyladenosine (m6A) on RNA. This innovation allows studying m6A

Keywords:
CRISPR-Cas9DemethylationMethylationN6-methyladenosineRNA targeting

More Related Videos

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.7K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.1K

Related Experiment Videos

Last Updated: Nov 3, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.9K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.7K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.1K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • RNA Biology

Background:

  • N6-methyladenosine (m6A) is a crucial epitranscriptomic modification impacting RNA metabolism.
  • The specific roles of m6A in different RNA regions are not fully understood.

Purpose of the Study:

  • To develop a novel CRISPR-Cas9-based system for targeted m6A modification on endogenous RNA.
  • To enable the study of regional m6A effects without altering the RNA sequence.

Main Methods:

  • Utilized a catalytically inactive CRISPR-Cas9 fused with engineered m6A modification enzymes.
  • Developed programmable m6A editors for targeted RNA methylation and demethylation.

Main Results:

  • Demonstrated the ability to precisely add or remove m6A at desired sites on endogenous RNA molecules.
  • Successfully created a tool for site-specific RNA modification.

Conclusions:

  • The developed CRISPR-Cas9 system provides a powerful tool for dissecting the functional impact of regional m6A modifications.
  • This technology expands the toolkit for RNA manipulation and epitranscriptomic research.