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Related Experiment Video

Updated: Nov 3, 2025

Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
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Gradient Tracking by Yeast GPCRs in a Microfluidics Chamber.

Sara Kimiko Suzuki1, Joshua B Kelley2, Timothy C Elston3,4,5

  • 1Curriculum in Bioinformatics and Computational Biology, UNC School of Medicine, Chapel Hill, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 4, 2021
PubMed
Summary

Microfluidic devices precisely control stimuli for single-cell analysis. Researchers used this technology with yeast to study cellular responses to pheromones, revealing insights into conserved signaling pathways.

Keywords:
Cell biologyG protein-coupled receptorGradientMicrofluidicsYeast

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Last Updated: Nov 3, 2025

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Area of Science:

  • Cell Biology
  • Biophysics
  • Systems Biology

Background:

  • Cells exist in dynamic environments not easily replicated in standard lab settings.
  • Microfluidics offers precise control over stimuli and quantitative single-cell data acquisition.
  • Yeast serves as a powerful model organism due to its genetic tractability and conserved signaling pathways.

Purpose of the Study:

  • To implement a microfluidic device for studying yeast cellular responses.
  • To investigate morphological and transcriptional changes in yeast exposed to GPCR ligand stimulation.
  • To analyze responses to both gradient and pulse administration of α-factor.

Main Methods:

  • Utilized a microfluidic device for controlled stimulus delivery.
  • Employed the yeast model organism for its genetic tractability.
  • Administered the peptide mating pheromone α-factor in gradient and pulse patterns.
  • Captured quantitative single-cell data on morphological and transcriptional responses.

Main Results:

  • Demonstrated the capability of microfluidics to mimic dynamic cellular environments.
  • Observed distinct morphological and transcriptional responses of yeast to α-factor stimulation.
  • Quantified single-cell responses to precise temporal and dosage control of the pheromone.

Conclusions:

  • Microfluidic devices are effective tools for dissecting complex cellular signaling.
  • Yeast's conserved G-protein coupled receptor (GPCR) pathway provides a relevant model for human cellular responses.
  • This approach enables detailed investigation of single-cell dynamics in response to specific stimuli.