[Enumeration and size of planktonic bacteria determined by image analysis coupled with epifluorescence]

The interest of the image analysis procedure is the time-saving in automated planktonic bacterial counting and sizing, with the possibility of manual visual field control at all times. Bacterial biomass (in number and volume) and bacterial projected area histograms were determined with a microcomputer. Performance limits of image-analysed epifluorescence microscopy were: camera sensitivity, considering the very low fluorescence levels on stained bacteria; pixel-micron conversion factor, and the impossibility of the apparatus distinguishing between bacteria and fluorescent small particles. This method is not of interest for counting sediment bacteria.