Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cocoa: a new mouse model for platelet storage pool deficiency.

E K Novak1, H O Sweet, M Prochazka

  • 1Roswell Park Memorial Institute, Department of Molecular and Cellular Biology, Buffalo, New York 14263.

British Journal of Haematology
|July 1, 1988
PubMed
Summary

A new mouse mutation, cocoa (coa), causes bleeding disorders due to platelet storage pool deficiency. This genetic mutation affects melanosomes and platelet dense granules, offering insights into human Hermansky-Pudlak syndrome.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advanced micro- & nanostructuring for enhanced biocompatibility of stainless steel by multi-beam and beamshaping technology.

Biomedical materials (Bristol, England)·2023
Same author

Urinary incontinence: vaginal delivery versus instrumental delivery.

Ceska gynekologie·2021
Same author

The effect of physiotherapy intervention on the load of the foot and low back pain in pregnancy.

Ceska gynekologie·2020
Same author

Echogenic foci in fetal heart from a pediatric cardiologists point of view.

Ceska gynekologie·2019
Same author

The risk of thromboembolism in relation to in vitro fertilization.

Ceska gynekologie·2019
Same author

HCG level after embryo transfer as a prognostic indicator of pregnancy finished with delivery.

Ceska gynekologie·2019

Area of Science:

  • Genetics
  • Hematology
  • Biochemistry

Background:

  • Platelet storage pool deficiency (SPD) is a bleeding disorder.
  • Mouse pigment mutations can affect platelet function.
  • Hermansky-Pudlak syndrome (HPS) involves defects in melanosomes and platelet dense granules.

Purpose of the Study:

  • To characterize a novel mouse pigment mutant, cocoa (coa).
  • To investigate the genetic, haematological, and biochemical properties of the cocoa mutation.
  • To determine if cocoa affects lysosomes, unlike other SPD mouse models.

Main Methods:

  • Genetic analysis to map the cocoa mutation to chromosome 3.
  • Haematological assays to assess bleeding time and platelet function.
  • Biochemical and electron microscopy analyses of platelet dense granules and lysosomal enzyme secretion.

Related Experiment Videos

Main Results:

  • The cocoa mutation is a recessive trait on chromosome 3, near Car-2.
  • Cocoa mice exhibit increased bleeding time and platelet storage pool deficiency (SPD).
  • Platelet dense granules show abnormal intragranular content but normal numbers; lysosomal enzyme secretion is unaffected.

Conclusions:

  • The cocoa mutation affects melanosomes and platelet dense granules, but not lysosomes.
  • This suggests shared synthesis or processing pathways between melanosomes and platelet dense granules.
  • Cocoa serves as a potential mouse model for specific forms of human Hermansky-Pudlak syndrome.